Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis

doi:10.1016/j.jplph.2012.11.007

	Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis
	Xiao, Jun 1; Zhang, Hong 1; Xing, Lijing ; Xu, Shujuan 1; Liu, Huanhuan 1; Chong, Kang ; Xu, Yunyuan
	2013
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
卷号	170 期号:4 页码:444-451
摘要	Histone acetylation is an important posttranslational modification associated with gene activation. In Arabidopsis, two MYST histone acetyltransferases HAM1 and HAM2 work redundantly to acetylate histone H4 lysine 5 (H4K5ace) in vitro. The double mutant ham1/ham2 is lethal, which suggests the critical role of HAM1 and HAM2 in development. Here, we used an artificial microRNA (amiRNA) strategy in Arabidopsis to uncover a novel function of HAM1 and HAM2. The amiRNA-HAM1/2 transgenic plants showed early flowering and reduced fertility. In addition, they responded normally to photoperiod, gibberellic acid treatment, and vernalization. The expression of flowering-repressor FLOWERING LOCUS C (FLC) and its homologues, MADS-box Affecting Flowering genes 3/4 (MAF3/4), were decreased in amiRNA-HAM1/2 lines. HAM1 overexpression caused late flowering and elevated expression of PLC and MAF3/4. Mutation of PLC almost rescued the late flowering with HAM1 overexpression, which suggests that HAM1 regulation of flowering time depended on PLC. Global H4 acetylation was decreased in amiRNA-HAM1/2 lines, but increased in HAM1-OE lines, which further confirmed the acetyltransferase activity of HAM1 in vivo. Chromatin immunoprecipitation revealed that H4 hyperacetylation and H4K5ace at FLC and MAF3/4 were less abundant in amiRNA-HAM1/2 lines than the wild type, but were enriched in HAM1-OE lines. Thus, HAM1 and HAM2 may affect flowering time by epigenetic modification of PLC and MAF3/4 chromatins at H4K5 acetylation. (C) 2012 Elsevier GmbH. All rights reserved.
关键词	Flowering Histone modification FLC HAM1/2
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2012.11.007
收录类别	SCI
语种	英语
WOS关键词	RNA-BINDING PROTEIN ; TRANSCRIPTIONAL ADAPTER ; LOCUS-C ; TIME ; GENE ; CHROMATIN ; ACETYLATION ; DIMETHYLATION ; VERNALIZATION ; ACTIVATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000316303900010
出版者	ELSEVIER GMBH
文献子类	Article
出版地	MUNICH
EISSN	1618-1328
资助机构	National Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Innovation Grant of the Chinese Academy of Sciences(Chinese Academy of Sciences)
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28000
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Jun,Zhang, Hong,Xing, Lijing,et al. Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis[J]. JOURNAL OF PLANT PHYSIOLOGY,2013,170(4):444-451.
APA	Xiao, Jun.,Zhang, Hong.,Xing, Lijing.,Xu, Shujuan.,Liu, Huanhuan.,...&Xu, Yunyuan.(2013).Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis.JOURNAL OF PLANT PHYSIOLOGY,170(4),444-451.
MLA	Xiao, Jun,et al."Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis".JOURNAL OF PLANT PHYSIOLOGY 170.4(2013):444-451.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S017616171200（1334KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文