Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins

doi:10.21273/JASHS.138.6.416

	Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins
	Wu, Ben-Hong ; Niu, Ning ; Li, Ji-Hu ; Li, Shao-Hua
	2013
发表期刊	JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
ISSN	0003-1062
卷号	138 期号:6 页码:416-427
摘要	The most obvious effects of a low leaf:fruit (LF) ratio [two leaves for one cluster per shoot (LF2)] on grape (Vitis vinifera) berries are suppressed anthocyanin biosynthesis in the berry skin, decreased berry weight and soluble solids concentration, and increased titratable acidity. In this study, proteins isolated from berry skins grown under low and high LF ratio conditions, LF2 and LF12, respectively, were characterized by two-dimensional gel electrophoresis coupled to mass spectrometry. A survey of approximate to 600 to 700 spots from berry skin yielded 77 proteins with differential expression between LF12 and LF2 treatments. Of these, the 59 proteins that were identified consisted of 47 proteins that were down-regulated and 12 that were up-regulated under LF2 conditions compared with LF12 conditions. Most proteins involved in metabolism, energy, transcription, protein synthesis, binding function, signal transduction, and cell defense were down-regulated in LF2 berries, whereas two important enzymes of anthocyanin biosynthesis, chalcone synthase and dihydroflavonol reductase, were not detected. Only a few proteins (e.g., two heat shock proteins related to protein fate and nutrient reservoir storage protein) were found to be up-regulated in LF2 berries. This suggested that, with the exception of secondary metabolism, many proteomic events may have an effect on anthocyanin synthesis in the skins responding to LF.
关键词	anthocyanin berry composition crop load two-dimensional gel electrophoresis Vitis vinifera
学科领域	Horticulture
DOI	10.21273/JASHS.138.6.416
收录类别	SCI
语种	英语
WOS关键词	VITIS-VINIFERA L ; FRUIT-QUALITY ; CROP LEVEL ; FLAVONOID BIOSYNTHESIS ; PRUNING SEVERITY ; SEASONAL-CHANGES ; TRAINING SYSTEM ; SHOOT DENSITY ; ORGANIC-ACIDS ; WINE QUALITY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000333230400002
出版者	AMER SOC HORTICULTURAL SCIENCE
文献子类	Article
出版地	ALEXANDRIA
EISSN	2327-9788
资助机构	CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Ministry of Agriculture(Gida Tarim Ve Hayvancilik Bakanligi)
作者邮箱	Li, Jihu/ABI-5460-2020 ; Wu, Benhong/Q-5328-2016
作品OA属性	gold
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28007
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Being Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wu, Ben-Hong,Niu, Ning,Li, Ji-Hu,et al. Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins[J]. JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE,2013,138(6):416-427.
APA	Wu, Ben-Hong,Niu, Ning,Li, Ji-Hu,&Li, Shao-Hua.(2013).Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins.JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE,138(6),416-427.
MLA	Wu, Ben-Hong,et al."Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins".JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE 138.6(2013):416-427.

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