Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites

doi:10.1007/s11816-012-0271-y

	Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites
	Guo, Yan-Wu ; Guo, Hui-Li ; Li, Xing ; Huang, Li-Li ; Zhang, Bo-Ning ; Pang, Xiao-Bin ; Liu, Ben-Ye ; Ma, Lan-Qing ; Wang, Hong 3
	2013
发表期刊	PLANT BIOTECHNOLOGY REPORTS
ISSN	1863-5466
卷号	7 期号:3 页码:371-381
摘要	In our recent work (Ma et al., in Planta 229(3):457-469, 2009a and 229(4):1077-1086, 2009b), two three-intron type III PKS genes, PcPKS1 and PcPKS2, were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is a three-intron chalcone synthase (CHS) gene, and PcPKS2 is found to be a three-intron benzalacetone synthase (BAS) gene. The regular CHS encoded by a single intron gene have not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3) and a stilbene synthase (STS) gene with three-intron (PcPKS5) were isolated and characterized by functional and phylogenetic analyses. In comparison with PcPKS1, a bifunctional enzyme with both CHS and BAS activity, the enzymatic product of recombinant PcPKS3 was naringenin, bis-noryangonin (BNY) and 4-coumaroyltriacetic acid lactone (CTAL) occurred as side products. The PcPKS5 synthesized resveratrol and a trace amount of naringenin from p-coumaroyl-CoA. To our knowledge, PcPKS5 is the first reported three-intron STS gene in flowering plants. In this work, we speculated that this involved a possible evolutionary route of plant-specific type III PKS superfamily in P. cuspidatum.
关键词	Chalcone synthase Gene evolution Polygonum cuspidatum Resveratrol Stilbene synthase Type III polyketide synthase
学科领域	Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1007/s11816-012-0271-y
收录类别	SCI
语种	英语
WOS关键词	CHALCONE SYNTHASE ; AROMATIC POLYKETIDE ; ENZYMATIC FORMATION ; 3-INTRON GENE ; BIOSYNTHESIS ; FAMILY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000321896900017
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Key Natural Science Foundation of Beijing Municipality(Beijing Natural Science Foundation) ; Foundation of Beijing Municipal Education Committee ; Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality
作者邮箱	Liu, Benye/AAF-4840-2020
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28021
专题	中科院植物分子生理学重点实验室
作者单位	1.[Guo, Yan-Wu 2.Li, Xing 3.Huang, Li-Li 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.[Guo, Hui-Li 6.China Agr Univ, Minist Agr PR China, Key Lab Urban Agr North, Beijing 102206, Peoples R China 7.Beijing Forestry Univ, Coll Forestry, Beijing 100083, Peoples R China 8.Beijing 2 Middle Sch, Beijing 100010, Peoples R China 9.Henan Univ, Inst Pharm, Kaifeng 475004, Peoples R China 10.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Guo, Yan-Wu,Guo, Hui-Li,Li, Xing,et al. Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites[J]. PLANT BIOTECHNOLOGY REPORTS,2013,7(3):371-381.
APA	Guo, Yan-Wu.,Guo, Hui-Li.,Li, Xing.,Huang, Li-Li.,Zhang, Bo-Ning.,...&Wang, Hong.(2013).Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites.PLANT BIOTECHNOLOGY REPORTS,7(3),371-381.
MLA	Guo, Yan-Wu,et al."Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites".PLANT BIOTECHNOLOGY REPORTS 7.3(2013):371-381.

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