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| Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth | |
| Zheng, Yiyan1; Xie, Yurong; Jiang, Yuxiang1; Qu, Xiaolu1; Huang, Shanjin2 | |
| 2013 | |
| Source Publication | PLANT CELL
 |
| ISSN | 1040-4651 |
| Volume | 25Issue:9Pages:3405-3423 |
| Abstract | Actin filaments are often arranged into higher-order structures, such as the longitudinal actin cables that generate the reverse fountain cytoplasmic streaming pattern present in pollen tubes. While several actin binding proteins have been implicated in the generation of these cables, the mechanisms that regulate their dynamic turnover remain largely unknown. Here, we show that Arabidopsis thaliana ACTIN-DEPOLYMERIZING FACTOR7 (ADF7) is required for turnover of longitudinal actin cables. In vitro biochemical analyses revealed that ADF7 is a typical ADF that prefers ADP-G-actin over ATP-G-actin. ADF7 inhibits nucleotide exchange on actin and severs filaments, but its filament severing and depolymerizing activities are less potent than those of the vegetative ADF1. ADF7 primarily decorates longitudinal actin cables in the shanks of pollen tubes. Consistent with this localization pattern, the severing frequency and depolymerization rate of filaments significantly decreased, while their maximum lifetime significantly increased, in adf7 pollen tube shanks. Furthermore, an ADF7-enhanced green fluorescent protein fusion with defective severing activity but normal G-actin binding activity could not complement adf7, providing compelling evidence that the severing activity of ADF7 is vital for its in vivo functions. These observations suggest that ADF7 evolved to promote turnover of longitudinal actin cables by severing actin filaments in pollen tubes. |
| Subject Area | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| DOI | 10.1105/tpc.113.117820 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | CELL-GROWTH ; GENE-EXPRESSION ; FACTORS ADF7 ; IN-VITRO ; DYNAMICS ; PROTEIN ; GERMINATION ; ADF/COFILIN ; PROFILIN ; COFILIN |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000326287100021 |
| Publisher | AMER SOC PLANT BIOLOGISTS |
| Subtype | Article |
| Publication Place | ROCKVILLE |
| EISSN | 1532-298X |
| Funding Organization | Ministry of Science and Technology of China(Ministry of Science and Technology, China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| Corresponding Author Email | Huang, Shanjin/M-4112-2017 |
| OA | Green Accepted, Bronze |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/28030 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China |
| Recommended Citation GB/T 7714 | Zheng, Yiyan,Xie, Yurong,Jiang, Yuxiang,et al. Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth[J]. PLANT CELL,2013,25(9):3405-3423. |
| APA | Zheng, Yiyan,Xie, Yurong,Jiang, Yuxiang,Qu, Xiaolu,&Huang, Shanjin.(2013).Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth.PLANT CELL,25(9),3405-3423. |
| MLA | Zheng, Yiyan,et al."Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth".PLANT CELL 25.9(2013):3405-3423. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Zheng-2013-Arabidops（1867KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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