Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth

doi:10.1105/tpc.113.117820

	Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth
	Zheng, Yiyan 1; Xie, Yurong ; Jiang, Yuxiang 1; Qu, Xiaolu 1; Huang, Shanjin 2
	2013
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	25 期号:9 页码:3405-3423
摘要	Actin filaments are often arranged into higher-order structures, such as the longitudinal actin cables that generate the reverse fountain cytoplasmic streaming pattern present in pollen tubes. While several actin binding proteins have been implicated in the generation of these cables, the mechanisms that regulate their dynamic turnover remain largely unknown. Here, we show that Arabidopsis thaliana ACTIN-DEPOLYMERIZING FACTOR7 (ADF7) is required for turnover of longitudinal actin cables. In vitro biochemical analyses revealed that ADF7 is a typical ADF that prefers ADP-G-actin over ATP-G-actin. ADF7 inhibits nucleotide exchange on actin and severs filaments, but its filament severing and depolymerizing activities are less potent than those of the vegetative ADF1. ADF7 primarily decorates longitudinal actin cables in the shanks of pollen tubes. Consistent with this localization pattern, the severing frequency and depolymerization rate of filaments significantly decreased, while their maximum lifetime significantly increased, in adf7 pollen tube shanks. Furthermore, an ADF7-enhanced green fluorescent protein fusion with defective severing activity but normal G-actin binding activity could not complement adf7, providing compelling evidence that the severing activity of ADF7 is vital for its in vivo functions. These observations suggest that ADF7 evolved to promote turnover of longitudinal actin cables by severing actin filaments in pollen tubes.
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1105/tpc.113.117820
收录类别	SCI
语种	英语
WOS关键词	CELL-GROWTH ; GENE-EXPRESSION ; FACTORS ADF7 ; IN-VITRO ; DYNAMICS ; PROTEIN ; GERMINATION ; ADF/COFILIN ; PROFILIN ; COFILIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000326287100021
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-298X
资助机构	Ministry of Science and Technology of China(Ministry of Science and Technology, China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
作者邮箱	Huang, Shanjin/M-4112-2017
作品OA属性	Green Accepted, Bronze
引用统计	被引频次：76[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28030
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Yiyan,Xie, Yurong,Jiang, Yuxiang,et al. Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth[J]. PLANT CELL,2013,25(9):3405-3423.
APA	Zheng, Yiyan,Xie, Yurong,Jiang, Yuxiang,Qu, Xiaolu,&Huang, Shanjin.(2013).Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth.PLANT CELL,25(9),3405-3423.
MLA	Zheng, Yiyan,et al."Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth".PLANT CELL 25.9(2013):3405-3423.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Zheng-2013-Arabidops（1867KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文