Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage

doi:10.1016/j.foodchem.2013.06.025

	Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage
	Zhu, Zhu 1; Liu, Ruiling 1; Li, Boqiang ; Tian, Shiping
	2013
发表期刊	FOOD CHEMISTRY
ISSN	0308-8146
卷号	141 期号:4 页码:3323-3328
摘要	Sugars are essential contributors to fruit flavour. Controlled atmosphere (CA) storage has been proved to be beneficial for maintaining harvested fruit quality. To explore regulatory mechanism of sugar metabolism in fruit stored in CA condition, we cloned several genes, encoding key enzymes, involved in sugar metabolism in apple fruit, and analyzed sugar contents, along with gene expression and enzyme activities in fruits stored in air and CA. The results indicated that CA could maintain higher contents of sugars, including sucrose, fructose and glucose. Expression levels of key genes, such as sucrose synthase (SS), sucrose phosphate synthase (SPS), fructokinase (FK) and hexokinase (HK), were shown to be correlated with the corresponding enzyme activities. We found that activities of neutral invertase (NI), vacuolar invertase (VI), FM and HK were inhibited, but SPS activity was promoted in apple fruit stored in CA, suggesting that CA storage could enhance sucrose synthesis and delay hydrolysis of sucrose and hexose. These findings provided molecular evidence to explain why higher sugar levels in harvested fruit are maintained under CA storage. (C) 2013 Elsevier Ltd. All rights reserved.
关键词	Sugar metabolism Key genes Apple fruit Controlled atmosphere
学科领域	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
DOI	10.1016/j.foodchem.2013.06.025
收录类别	SCI
语种	英语
WOS关键词	SUCROSE-PHOSPHATE SYNTHASE ; OXYGEN ; PHYSIOLOGY ; INVERTASE ; QUALITY ; PLANTS ; O-2
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000324848000005
出版者	ELSEVIER SCI LTD
文献子类	Article
出版地	OXFORD
EISSN	1873-7072
资助机构	National Basic Research Program of China (973 Program)(National Basic Research Program of China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
作者邮箱	Li, Boqiang/L-8483-2019
引用统计	被引频次：64[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28034
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Zhu,Liu, Ruiling,Li, Boqiang,et al. Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage[J]. FOOD CHEMISTRY,2013,141(4):3323-3328.
APA	Zhu, Zhu,Liu, Ruiling,Li, Boqiang,&Tian, Shiping.(2013).Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage.FOOD CHEMISTRY,141(4),3323-3328.
MLA	Zhu, Zhu,et al."Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage".FOOD CHEMISTRY 141.4(2013):3323-3328.

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