Expression and Localization of Amorpha-4,11-diene Synthase in Artemisia annua L.

doi:10.1007/s11105-012-0472-0

	Expression and Localization of Amorpha-4,11-diene Synthase in Artemisia annua L.
	Pu, Gao-Bin ; Ma, Dong-Ming ; Wang, Hong 2; Ye, He-Chun ; Liu, Ben-Ye
	2013
发表期刊	PLANT MOLECULAR BIOLOGY REPORTER
ISSN	0735-9640
卷号	31 期号:1 页码:32-37
摘要	Artemisia annua L. is the only commercial source of the widely used antimalarial compound artemisinin. Biosynthesis of artemisinin has been proposed to take place in glandular trichomes. The first committed step in the conversion of farnesyl diphosphate (FDP) to artemisinin is conducted by the amorpha-4,11-diene synthase (ADS). To explore the organ-specific and developmental distributions of ADS, rabbit polyclonal antibodies were raised against recombinant ADS produced in Escherichia coli from the corresponding A. annua cDNA. Protein gel blot analysis of different A. annua organs showed that ADS was most abundant in young leaves and flower buds. Minor amounts of ADS were found in mature leaves. These findings were generally consistent with the analysis of the transcript level of the ADS gene. Immunolocalization of ADS showed strong positive staining in apical meristems, young leaves and glandular trichomes. No staining was observed in other cells of the leaf. The whole mount hybridization revealed that ADS was not expressed in all glandular trichomes of mature leaves. Specific staining of ADS could be detected in about 10-40 % of glandular trichomes.
关键词	Artemisia annua L. Glandular trichome Amorpha-4,11-diene synthase Artemisinin Immunocytochemistry Immunohistochemistry
学科领域	Biochemical Research Methods ; Plant Sciences
DOI	10.1007/s11105-012-0472-0
收录类别	SCI
语种	英语
WOS关键词	GLANDULAR TRICHOMES ; KEY ENZYME ; MOLECULAR-CLONING ; ESSENTIAL OIL ; BIOSYNTHESIS ; PLANTS ; TISSUES ; GENES ; HOP
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000313412900004
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
资助机构	National High-tech R&D Program (863) of the Ministry of Sciences and Technology, China(National High Technology Research and Development Program of China) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
作者邮箱	Liu, Benye/AAF-4840-2020
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28039
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Shandong Yingcai Univ, Jinan 250104, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Pu, Gao-Bin,Ma, Dong-Ming,Wang, Hong,et al. Expression and Localization of Amorpha-4,11-diene Synthase in Artemisia annua L.[J]. PLANT MOLECULAR BIOLOGY REPORTER,2013,31(1):32-37.
APA	Pu, Gao-Bin,Ma, Dong-Ming,Wang, Hong,Ye, He-Chun,&Liu, Ben-Ye.(2013).Expression and Localization of Amorpha-4,11-diene Synthase in Artemisia annua L..PLANT MOLECULAR BIOLOGY REPORTER,31(1),32-37.
MLA	Pu, Gao-Bin,et al."Expression and Localization of Amorpha-4,11-diene Synthase in Artemisia annua L.".PLANT MOLECULAR BIOLOGY REPORTER 31.1(2013):32-37.

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