Surface Plasmon Resonance Detection of Transgenic Cry1Ac Cotton (Gossypium spp.)

doi:10.1021/jf3050439

	Surface Plasmon Resonance Detection of Transgenic Cry1Ac Cotton (Gossypium spp.)
	Zhao, Zhuoya 1; Chen, Yanshan 1; Xu, Wenzhong ; Ma, Mi
	2013
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
卷号	61 期号:12 页码:2964-2969
摘要	The detection and identification of genetically modified (GM) plants are challenging issues that have arisen from the potential negative impacts of extensive cultivation of transgenic plants. The screening process is a long-term focus and needs specific detection strategies. Surface plasmon resonance (SPR) has been used to detect a variety of biomolecules including proteins and nucleic acids due to its ability to monitor specific intermolecular interactions. In the present study, two high-throughput, label-free, and specific methods based on SPR technology were developed to detect transgenic CrylAc cotton (Gossypium spp.) by separately targeting protein and DNA. In the protein-based detection system, monoclonal anti-Cry1Ac antibodies were immobilized on the surface of a CMS sensor chip. Conventional cotton samples were used to define the detection threshold. Transgenic cotton was easily identified within 5 min per sample. For the DNA-based model, a 25-mer biotinylated oligonucleotide probe was immobilized on an SA sensor chip. PCR products of CrylAc (230 bp) were used to investigate the reaction conditions. The sensitivity of the constructed sensor chip was identified at concentrations as low as 0.1 nM based on its complementary base pairing.
关键词	surface plasmon resonance Cry1Ac cotton protein-based detection DNA-based detection
学科领域	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
DOI	10.1021/jf3050439
收录类别	SCI
语种	英语
WOS关键词	GENETICALLY-MODIFIED ORGANISMS ; CHAIN-REACTION METHODS ; MODIFIED RICE ; BT COTTON ; MAIZE ; GENE ; CROPS ; ELISA ; PCR ; SEQUENCES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000317031800009
出版者	AMER CHEMICAL SOC
文献子类	Article
出版地	WASHINGTON
资助机构	National Transgenic Plant Special Fund ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
作者邮箱	Chen, Yanshan/F-1830-2017
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28046
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Zhuoya,Chen, Yanshan,Xu, Wenzhong,et al. Surface Plasmon Resonance Detection of Transgenic Cry1Ac Cotton (Gossypium spp.)[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2013,61(12):2964-2969.
APA	Zhao, Zhuoya,Chen, Yanshan,Xu, Wenzhong,&Ma, Mi.(2013).Surface Plasmon Resonance Detection of Transgenic Cry1Ac Cotton (Gossypium spp.).JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,61(12),2964-2969.
MLA	Zhao, Zhuoya,et al."Surface Plasmon Resonance Detection of Transgenic Cry1Ac Cotton (Gossypium spp.)".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 61.12(2013):2964-2969.

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