Chloroplast Small Heat Shock Protein HSP21 Interacts with Plastid Nucleoid Protein pTAC5 and Is Essential for Chloroplast Development in Arabidopsis under Heat Stress

doi:10.1105/tpc.113.111229

IB-CAS > 中科院光生物学重点实验室

	Chloroplast Small Heat Shock Protein HSP21 Interacts with Plastid Nucleoid Protein pTAC5 and Is Essential for Chloroplast Development in Arabidopsis under Heat Stress
	Zhong, Linlin 1; Zhou, Wen 1; Wang, Haijun 1; Ding, Shunhua ; Lu, Qingtao; Wen, Xiaogang ; Peng, Lianwei ; Zhang, Lixin2 ; Lu, Congming 2
	2013
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	25 期号:8 页码:2925-2943
摘要	Compared with small heat shock proteins (sHSPs) in other organisms, those in plants are the most abundant and diverse. However, the molecular mechanisms by which sHSPs are involved in cell protection remain unknown. Here, we characterized the role of HSP21, a plastid nucleoid-localized sHSP, in chloroplast development under heat stress. We show that an Arabidopsis thaliana knockout mutant of HSP21 had an ivory phenotype under heat stress. Quantitative real-time RT-PCR, run-on transcription, RNA gel blot, and polysome association analyses demonstrated that HSP21 is involved in plastid-encoded RNA polymerase (PEP)-dependent transcription. We found that the plastid nucleoid protein pTAC5 was an HSP21 target. pTAC5 has a C4-type zinc finger similar to that of Escherichia coli DnaJ and zinc-dependent disulfide isomerase activity. Reduction of pTAC5 expression by RNA interference led to similar phenotypic effects as observed in hsp21. HSP21 and pTAC5 formed a complex that was associated mainly with the PEP complex. HSP21 and pTAC5 were associated with the PEP complex not only during transcription initiation, but also during elongation and termination. Our results suggest that HSP21 and pTAC5 are required for chloroplast development under heat stress by maintaining PEP function.
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1105/tpc.113.111229
收录类别	SCI
语种	英语
WOS关键词	ENCODED RNA-POLYMERASE ; DISULFIDE OXIDOREDUCTASE ACTIVITIES ; DNA-BINDING PROTEIN ; GENE-EXPRESSION ; PHOTOSYSTEM-II ; OXIDATIVE STRESS ; HIGHER-PLANTS ; CHLOROPHYLL BIOSYNTHESIS ; QUATERNARY DYNAMICS ; CHAPERONE FUNCTION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000324920500016
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
资助机构	State Key Basic Research and Development Plan of China(National Basic Research Program of China) ; Solar Energy Initiative of the Chinese Academy of Sciences(Chinese Academy of Sciences)
作品OA属性	Green Accepted
引用统计	被引频次：155[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28049
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhong, Linlin,Zhou, Wen,Wang, Haijun,et al. Chloroplast Small Heat Shock Protein HSP21 Interacts with Plastid Nucleoid Protein pTAC5 and Is Essential for Chloroplast Development in Arabidopsis under Heat Stress[J]. PLANT CELL,2013,25(8):2925-2943.
APA	Zhong, Linlin.,Zhou, Wen.,Wang, Haijun.,Ding, Shunhua.,Lu, Qingtao.,...&Lu, Congming.(2013).Chloroplast Small Heat Shock Protein HSP21 Interacts with Plastid Nucleoid Protein pTAC5 and Is Essential for Chloroplast Development in Arabidopsis under Heat Stress.PLANT CELL,25(8),2925-2943.
MLA	Zhong, Linlin,et al."Chloroplast Small Heat Shock Protein HSP21 Interacts with Plastid Nucleoid Protein pTAC5 and Is Essential for Chloroplast Development in Arabidopsis under Heat Stress".PLANT CELL 25.8(2013):2925-2943.

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