Small GTPase Sar1 is crucial for proglutelin and -globulin export from the endoplasmic reticulum in rice endosperm

doi:10.1093/jxb/ert128

	Small GTPase Sar1 is crucial for proglutelin and -globulin export from the endoplasmic reticulum in rice endosperm
	Tian, Lihong ; Dai, Ling Ling ; Yin, Zhi Jie ; Fukuda, Masako 1; Kumamaru, Toshihiro 1; Dong, Xiang Bai ; Xu, Xiu Ping ; Qu, Le Qing
	2013
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	64 期号:10 页码:2831-2845
摘要	Rice seed storage proteins glutelin and -globulin are synthesized in the endoplasmic reticulum (ER) and deposited in protein storage vacuoles (PSVs). Sar1, a small GTPase, acts as a molecular switch to regulate the assembly of coat protein complex II, which exports secretory protein from the ER to the Golgi apparatus. To reveal the route by which glutelin and -globulin exit the ER, four putative Sar1 genes (OsSar1a/b/c/d) were cloned from rice, and transgenic rice were generated with Sar1 overexpressed or suppressed by RNA interference (RNAi) specifically in the endosperm under the control of the rice glutelin promoter. Overexpression or suppression of any OsSar1 did not alter the phenotype. However, simultaneous knockdown of OsSar1a/b/c resulted in floury and shrunken seeds, with an increased level of glutelin precursor and decreased level of the mature - and -subunit. OsSar1abc RNAi endosperm generated numerous, spherical, novel protein bodies with highly electron-dense matrixes containing both glutelin and -globulin. Notably, the novel protein bodies were surrounded by ribosomes, showing that they were derived from the ER. Some of the ER-derived dense protein bodies were attached to a blebbing structure containing prolamin. These results indicated that OsSar1a/b/c play a crucial role in storage proteins exiting from the ER, with functional redundancy in rice endosperm, and glutelin and -globulin transported together from the ER to the Golgi apparatus by a pathway mediated by coat protein complex II.
关键词	Endoplasmic reticulum OsSar1 protein body protein intracellular transport protein storage vacuole rice storage proteins
学科领域	Plant Sciences
DOI	10.1093/jxb/ert128
收录类别	SCI
语种	英语
WOS关键词	SEED STORAGE PROTEINS ; ORYZA-SATIVA-L ; GOLGI-APPARATUS ; MESSENGER-RNA ; COPII ; TRANSPORT ; EFFICIENT ; PLANT ; ER ; EXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000321055700015
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Program of Transgenic Variety Development of China ; National High Technology Research and Development Program (863)(National High Technology Research and Development Program of China)
作者邮箱	Tian, li/HQY-8623-2023
作品OA属性	hybrid, Green Submitted, Green Accepted, Green Published
引用统计	被引频次：51[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28055
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Kyushu Univ, Fac Agr, Fukuoka 8128581, Japan
推荐引用方式 GB/T 7714	Tian, Lihong,Dai, Ling Ling,Yin, Zhi Jie,et al. Small GTPase Sar1 is crucial for proglutelin and -globulin export from the endoplasmic reticulum in rice endosperm[J]. JOURNAL OF EXPERIMENTAL BOTANY,2013,64(10):2831-2845.
APA	Tian, Lihong.,Dai, Ling Ling.,Yin, Zhi Jie.,Fukuda, Masako.,Kumamaru, Toshihiro.,...&Qu, Le Qing.(2013).Small GTPase Sar1 is crucial for proglutelin and -globulin export from the endoplasmic reticulum in rice endosperm.JOURNAL OF EXPERIMENTAL BOTANY,64(10),2831-2845.
MLA	Tian, Lihong,et al."Small GTPase Sar1 is crucial for proglutelin and -globulin export from the endoplasmic reticulum in rice endosperm".JOURNAL OF EXPERIMENTAL BOTANY 64.10(2013):2831-2845.

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