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| Small GTPase Sar1 is crucial for proglutelin and -globulin export from the endoplasmic reticulum in rice endosperm | |
| Tian, Lihong; Dai, Ling Ling; Yin, Zhi Jie; Fukuda, Masako1; Kumamaru, Toshihiro1; Dong, Xiang Bai; Xu, Xiu Ping; Qu, Le Qing | |
| 2013 | |
| Source Publication | JOURNAL OF EXPERIMENTAL BOTANY
 |
| ISSN | 0022-0957 |
| Volume | 64Issue:10Pages:2831-2845 |
| Abstract | Rice seed storage proteins glutelin and -globulin are synthesized in the endoplasmic reticulum (ER) and deposited in protein storage vacuoles (PSVs). Sar1, a small GTPase, acts as a molecular switch to regulate the assembly of coat protein complex II, which exports secretory protein from the ER to the Golgi apparatus. To reveal the route by which glutelin and -globulin exit the ER, four putative Sar1 genes (OsSar1a/b/c/d) were cloned from rice, and transgenic rice were generated with Sar1 overexpressed or suppressed by RNA interference (RNAi) specifically in the endosperm under the control of the rice glutelin promoter. Overexpression or suppression of any OsSar1 did not alter the phenotype. However, simultaneous knockdown of OsSar1a/b/c resulted in floury and shrunken seeds, with an increased level of glutelin precursor and decreased level of the mature - and -subunit. OsSar1abc RNAi endosperm generated numerous, spherical, novel protein bodies with highly electron-dense matrixes containing both glutelin and -globulin. Notably, the novel protein bodies were surrounded by ribosomes, showing that they were derived from the ER. Some of the ER-derived dense protein bodies were attached to a blebbing structure containing prolamin. These results indicated that OsSar1a/b/c play a crucial role in storage proteins exiting from the ER, with functional redundancy in rice endosperm, and glutelin and -globulin transported together from the ER to the Golgi apparatus by a pathway mediated by coat protein complex II. |
| Keyword | Endoplasmic reticulum OsSar1 protein body protein intracellular transport protein storage vacuole rice storage proteins |
| Subject Area | Plant Sciences |
| DOI | 10.1093/jxb/ert128 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | SEED STORAGE PROTEINS ; ORYZA-SATIVA-L ; GOLGI-APPARATUS ; MESSENGER-RNA ; COPII ; TRANSPORT ; EFFICIENT ; PLANT ; ER ; EXPRESSION |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000321055700015 |
| Publisher | OXFORD UNIV PRESS |
| Subtype | Article |
| Publication Place | OXFORD |
| EISSN | 1460-2431 |
| Funding Organization | Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Program of Transgenic Variety Development of China ; National High Technology Research and Development Program (863)(National High Technology Research and Development Program of China) |
| Corresponding Author Email | Tian, li/HQY-8623-2023 |
| OA | hybrid, Green Submitted, Green Accepted, Green Published |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/28055 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Kyushu Univ, Fac Agr, Fukuoka 8128581, Japan |
| Recommended Citation GB/T 7714 | Tian, Lihong,Dai, Ling Ling,Yin, Zhi Jie,et al. Small GTPase Sar1 is crucial for proglutelin and -globulin export from the endoplasmic reticulum in rice endosperm[J]. JOURNAL OF EXPERIMENTAL BOTANY,2013,64(10):2831-2845. |
| APA | Tian, Lihong.,Dai, Ling Ling.,Yin, Zhi Jie.,Fukuda, Masako.,Kumamaru, Toshihiro.,...&Qu, Le Qing.(2013).Small GTPase Sar1 is crucial for proglutelin and -globulin export from the endoplasmic reticulum in rice endosperm.JOURNAL OF EXPERIMENTAL BOTANY,64(10),2831-2845. |
| MLA | Tian, Lihong,et al."Small GTPase Sar1 is crucial for proglutelin and -globulin export from the endoplasmic reticulum in rice endosperm".JOURNAL OF EXPERIMENTAL BOTANY 64.10(2013):2831-2845. |
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| Tian-2013-Small GTPa（5507KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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