Identification and Dynamic Regulation of microRNAs Involved in Salt Stress Responses in Functional Soybean Nodules by High-Throughput Sequencing

doi:10.3390/ijms14022717

	Identification and Dynamic Regulation of microRNAs Involved in Salt Stress Responses in Functional Soybean Nodules by High-Throughput Sequencing
	Dong, Zhanghui 1,2; Shi, Lei; Wang, Yanwei 3; Chen, Liang ; Cai, Zhaoming 1; Wang, Youning ; Jin, Jingbo4 ; Li, Xia
	2013
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1422-0067
卷号	14 期号:2 页码:2717-2738
摘要	Both symbiosis between legumes and rhizobia and nitrogen fixation in functional nodules are dramatically affected by salt stress. Better understanding of the molecular mechanisms that regulate the salt tolerance of functional nodules is essential for genetic improvement of nitrogen fixation efficiency. microRNAs (miRNAs) have been implicated in stress responses in many plants and in symbiotic nitrogen fixation (SNF) in soybean. However, the dynamic regulation of miRNAs in functioning nodules during salt stress response remains unknown. We performed deep sequencing of miRNAs to understand the miRNA expression profile in normal or salt stressed-soybean mature nodules. We identified 110 known miRNAs belonging to 61 miRNA families and 128 novel miRNAs belonging to 64 miRNA families. Among them, 104 miRNAs were dramatically differentially expressed (>2-fold or detected only in one library) during salt stress. qRT-PCR analysis of eight miRNAs confirmed that these miRNAs were dynamically regulated in response to salt stress in functional soybean nodules. These data significantly increase the number of miRNAs known to be expressed in soybean nodules, and revealed for the first time a dynamic regulation of miRNAs during salt stress in functional nodules. The findings suggest great potential for miRNAs in functional soybean nodules during salt stress.
关键词	functional nodules miRNA nitrogen fixation salt stress soybean
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/ijms14022717
收录类别	SCI
语种	英语
WOS关键词	NITROGEN-FIXATION ; TRANSCRIPTION FACTORS ; ION HOMEOSTASIS ; NACL STRESS ; SMALL RNAS ; NODULATION ; DROUGHT ; MIRNAS ; EXPRESSION ; NUTRITION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000315397900027
出版者	MDPI
文献子类	Article
出版地	BASEL
资助机构	Ministry of Agriculture of the People's Republic of China(Ministry of Agriculture & Rural Affairs) ; NSFC of CAS ; Hundred Talents Program of CAS(Chinese Academy of Sciences)
作者邮箱	li, xia/HHZ-7468-2022 ; Cai, Zhaoming/HNI-0192-2023
作品OA属性	Green Submitted, gold, Green Published
引用统计	被引频次：54[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28061
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Agr Resources Res, Key State Lab Plant Cell & Chromosome Engn, Shijiazhuang 050021, Hebei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Shijiazhuang Acad Agr & Forestry Sci, Shijiazhuang 050041, Hebei, Peoples R China 4.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Key Lab Genet & Breeding Forest Trees & Ornamenta, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Dong, Zhanghui,Shi, Lei,Wang, Yanwei,et al. Identification and Dynamic Regulation of microRNAs Involved in Salt Stress Responses in Functional Soybean Nodules by High-Throughput Sequencing[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2013,14(2):2717-2738.
APA	Dong, Zhanghui.,Shi, Lei.,Wang, Yanwei.,Chen, Liang.,Cai, Zhaoming.,...&Li, Xia.(2013).Identification and Dynamic Regulation of microRNAs Involved in Salt Stress Responses in Functional Soybean Nodules by High-Throughput Sequencing.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,14(2),2717-2738.
MLA	Dong, Zhanghui,et al."Identification and Dynamic Regulation of microRNAs Involved in Salt Stress Responses in Functional Soybean Nodules by High-Throughput Sequencing".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 14.2(2013):2717-2738.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Dong-2013-Identifica（759KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文