Spectral and functional studies on siphonaxanthin-type light-harvesting complex of photosystem II from Bryopsis corticulans

doi:10.1007/s11120-013-9808-3

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	Spectral and functional studies on siphonaxanthin-type light-harvesting complex of photosystem II from Bryopsis corticulans
	Wang, Wenda 1; Qin, Xiaochun ; Sang, Min ; Chen, Dongqin 1; Wang, Kebin ; Lin, Rongchen ; Lu, Congming ; Shen, Jian-Ren; Kuang, Tingyun
	2013
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	117 期号:1-3 页码:267-279
摘要	Carotenoids with conjugated carbonyl groups possess special photophysical properties which have been studied in some water-soluble light-harvesting proteins (Polivka and Sundstrom, Chem Rev 104:2021-2071, 2004). However, siphonaxanthin-type light-harvesting complexes of photosystem II (LHCII) in siphonous green alga have received fewer studies. In the present study, we determined sequences of genes for several Bryopsis corticulans Lhcbm proteins, which showed that they belong to the group of major LHCII and diverged early from green algae and higher plants. Analysis of pigment composition indicated that this siphonaxanthin-type LHCII contained in total 3 siphonaxanthin and siphonein but no lutein and violaxanthin. In addition, 2 chlorophylls a in higher plant LHCII were replaced by chlorophyll b. These changes led to an increased absorption in green and blue-green light region compared with higher plant LHCII. The binding sites for chlorophylls, siphonaxanthin, and siphonein were suggested based on the structural comparison with that of higher plant LHCII. All of the ligands for the chlorophylls were completely conserved, suggesting that the two chlorophylls b were replaced by chlorophyll a without changing their binding sites in higher plant LHCII. Comparisons of the absorption spectra of isolated siphonaxanthin and siphonein in different organic solutions and the effect of heat treatment suggested that these pigments existed in a low hydrophobic protein environment, leading to an enhancement of light harvesting in the green light region. This low hydrophobic protein environment was maintained by the presence of more serine and threonine residues in B. corticulans LHCII. Finally, esterization of siphonein may also contribute to the enhanced harvesting of green light.
关键词	Bryopsis corticulans Light-harvesting proteins LHCII Pigments Siphonaxanthin Carotenoids
学科领域	Plant Sciences
DOI	10.1007/s11120-013-9808-3
收录类别	SCI
语种	英语
WOS关键词	EXCITATION RELAXATION DYNAMICS ; CHLOROPHYLL-PROTEIN COMPLEXES ; SIPHONOUS GREEN-ALGA ; ENERGY-TRANSFER ; PERIDININ-CHLOROPHYLL ; EVOLUTION ; ANTENNA ; STATE ; RECONSTITUTION ; DIVERSITY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000326604900019
出版者	SPRINGER
Special Issue	SI
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5079
资助机构	National Basic Research Program of China (973 Program)(National Basic Research Program of China) ; Grants-in-Aid for Scientific Research(Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI))
作者邮箱	Shen, Jian-Ren/B-2558-2011
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28063
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100093, Peoples R China 3.Okayama Univ, Fac Sci, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan
推荐引用方式 GB/T 7714	Wang, Wenda,Qin, Xiaochun,Sang, Min,et al. Spectral and functional studies on siphonaxanthin-type light-harvesting complex of photosystem II from Bryopsis corticulans[J]. PHOTOSYNTHESIS RESEARCH,2013,117(1-3):267-279.
APA	Wang, Wenda.,Qin, Xiaochun.,Sang, Min.,Chen, Dongqin.,Wang, Kebin.,...&Kuang, Tingyun.(2013).Spectral and functional studies on siphonaxanthin-type light-harvesting complex of photosystem II from Bryopsis corticulans.PHOTOSYNTHESIS RESEARCH,117(1-3),267-279.
MLA	Wang, Wenda,et al."Spectral and functional studies on siphonaxanthin-type light-harvesting complex of photosystem II from Bryopsis corticulans".PHOTOSYNTHESIS RESEARCH 117.1-3(2013):267-279.

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