Overexpression of sheepgrass R1-MYB transcription factor LcMYB1 confers salt tolerance in transgenic Arabidopsis

doi:10.1016/j.plaphy.2013.05.025

	Overexpression of sheepgrass R1-MYB transcription factor LcMYB1 confers salt tolerance in transgenic Arabidopsis
	Cheng, Liqin ; Li, Xiaoxia 1; Huang, Xin 1; Ma, Tian 2; Liang, Ye 1; Ma, Xingyong 1; Peng, Xianjun 1; Jia, Junting 1; Chen, Shuangyan ; Chen, Yan 4; Deng, Bo 3; Liu, Gongshe
	2013
发表期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
卷号	70 页码:252-260
摘要	Sheepgrass [Leymus chinensis (Trin.) Tzvel.] is a dominant, rhizomatous grass that has extensive plasticity in adapting to various harsh environments. Based on data from 454 high-throughput sequencing (GS FIX) exposure to salt stress, an unknown functional MYB-related gene LcMYB1 was identified from sheepgrass. Tissue specific expression profiles showed that the LcMYB1 gene was expressed ubiquitously in different tissues, with higher expression levels observed in the rhizome and panicle. The expression of LcMYB1 was induced obviously by high salt, drought and abscisic acid and was induced slightly by cold. A fusion protein of LcMYB1 with green fluorescent protein (GFP) was localized to the nucleus, and yeast one-hybrid analysis indicated that LcMYB1 was an activator of transcriptional activity. LcMYB1-overexpressing plants were more tolerant to salt stress than WT plants. The amounts of proline and soluble sugars were higher in transgenic Arabidopsis than in WT plants under salt stress conditions. The overexpression of LcMYB1 enhanced the expression levels of P5CS1 and inhibited other salt stress response gene markers. These findings demonstrate that LcMYB1 influences the intricate salt stress response signaling networks by promoting different pathways than the classical DREB1A- and MYB2-mediated signaling pathway. Additionally, LcMYB1 is a promising gene resource for improving salinity tolerance in crops. (c) 2013 Elsevier Masson SAS. All rights reserved.
关键词	Sheepgrass MYB-related LcMYB1 Salt stress
学科领域	Plant Sciences
DOI	10.1016/j.plaphy.2013.05.025
收录类别	SCI
语种	英语
WOS关键词	STRESS TOLERANCE ; PROLINE ACCUMULATION ; SIGNAL-TRANSDUCTION ; ENHANCED TOLERANCE ; ECTOPIC EXPRESSION ; GENE-EXPRESSION ; DROUGHT ; RICE ; SALINITY ; RESPONSES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000323355700029
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
文献子类	Article
出版地	ISSY-LES-MOULINEAUX
EISSN	1873-2690
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National High Technology and Research Development Program of China ('863' project)(National High Technology Research and Development Program of China) ; Chinese Ministry of Agriculture Research Program ; Ningxia agricultural comprehensive development land management science and technology promotion project
引用统计	被引频次：56[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28074
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Ning Xia Univ, Coll Agr, Ningxia 750021, Peoples R China 4.China Agr Univ, Coll Anim Sci & Technol, Dept Grassland Sci, Beijing 100193, Peoples R China 5.Ningxia Agr Comprehens Dev Off, Yinchuan 750001, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Liqin,Li, Xiaoxia,Huang, Xin,et al. Overexpression of sheepgrass R1-MYB transcription factor LcMYB1 confers salt tolerance in transgenic Arabidopsis[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2013,70:252-260.
APA	Cheng, Liqin.,Li, Xiaoxia.,Huang, Xin.,Ma, Tian.,Liang, Ye.,...&Liu, Gongshe.(2013).Overexpression of sheepgrass R1-MYB transcription factor LcMYB1 confers salt tolerance in transgenic Arabidopsis.PLANT PHYSIOLOGY AND BIOCHEMISTRY,70,252-260.
MLA	Cheng, Liqin,et al."Overexpression of sheepgrass R1-MYB transcription factor LcMYB1 confers salt tolerance in transgenic Arabidopsis".PLANT PHYSIOLOGY AND BIOCHEMISTRY 70(2013):252-260.

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