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| The Cyclophilin CYP20-2 Modulates the Conformation of BRASSINAZOLE-RESISTANT1, Which Binds the Promoter of FLOWERING LOCUS D to Regulate Flowering in Arabidopsis | |
| Zhang, Yuanyuan1; Li, Beibei; Xu, Yunyuan; Li, Heng2; Li, Shanshan2; Zhang, Dajian1; Mao, Zhiwei; Guo, Siyi; Yang, Chunhong3; Weng, Yuxiang2; Chong, Kang | |
| 2013 | |
| 发表期刊 | PLANT CELL
 |
| ISSN | 1040-4651 |
| 卷号 | 25期号:7页码:2504-2521 |
| 摘要 | Brassinosteroids (BRs) regulate many physiological processes during plant development, including flowering. However, little is known about the components of BR signaling that mediate flowering. Here, we report that BRASSINAZOLE-RESISTANT1 (BZR1), the conformation of which is altered by a cyclophilin (CYP20-2), binds cis-elements in the FLOWERING LOCUS D (FLD) promoter to regulate flowering. Both bzr1-1D and fld-4 showed delayed flowering. Electrophoretic mobility shift assay and chromatin immunoprecipitation revealed that BZR1 bound to a putative BR response cis-element and suppressed the expression of FLD. Overexpression of FLD partially rescued the late flowering of pBZR1:mBZR1(Pro234-Leu)-CFP (mx3). Yeast two-hybrid and pull-down assays demonstrated that BZR1 interacts with CYP20-2. Arabidopsis thaliana CYP20-2 had greater peptidyl-prolyl cis-trans isomerase activity than did wheat (Triticum aestivum) CYP20-2. Fourier transform infrared spectroscopy revealed conformation changes in BZR1, dependent on interaction with CYP20-2. Due to differences in activity and substrate preference between CYP20-2 proteins from wheat and Arabidopsis, At-CYP20-2-overexpressing lines showed earlier flowering, whereas Ta CYP20-2 lines flowered later. Immunoblot and chromatin immunoprecipitation assays showed that histone H3 trimethyl Lys4 and H3 acetylation levels were negatively correlated with the transcription of FLD (a putative histone demethylase) in various lines. Therefore, a conformational change of BZR1 mediated by CYP20-2 causes altered flowering through modulation of FLD expression. |
| 学科领域 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| DOI | 10.1105/tpc.113.110296 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | BRASSINOSTEROID SIGNAL-TRANSDUCTION ; GENE-EXPRESSION ; TRANSCRIPTION FACTOR ; INSENSITIVE MUTANT ; PLANT-GROWTH ; TIME ; PROTEIN ; THALIANA ; BES1 ; BZR1 |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000323650900012 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 文献子类 | Article |
| 出版地 | ROCKVILLE |
| EISSN | 1532-298X |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Foundation for Innovative Research Groups of the National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 作者邮箱 | Li, Shanshan/HLH-7747-2023 ; Guo, Siyi/AAT-4247-2020 |
| 作品OA属性 | Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/28075 |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yuanyuan,Li, Beibei,Xu, Yunyuan,et al. The Cyclophilin CYP20-2 Modulates the Conformation of BRASSINAZOLE-RESISTANT1, Which Binds the Promoter of FLOWERING LOCUS D to Regulate Flowering in Arabidopsis[J]. PLANT CELL,2013,25(7):2504-2521. |
| APA | Zhang, Yuanyuan.,Li, Beibei.,Xu, Yunyuan.,Li, Heng.,Li, Shanshan.,...&Chong, Kang.(2013).The Cyclophilin CYP20-2 Modulates the Conformation of BRASSINAZOLE-RESISTANT1, Which Binds the Promoter of FLOWERING LOCUS D to Regulate Flowering in Arabidopsis.PLANT CELL,25(7),2504-2521. |
| MLA | Zhang, Yuanyuan,et al."The Cyclophilin CYP20-2 Modulates the Conformation of BRASSINAZOLE-RESISTANT1, Which Binds the Promoter of FLOWERING LOCUS D to Regulate Flowering in Arabidopsis".PLANT CELL 25.7(2013):2504-2521. |
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| Zhang-2013-The Cyclo（2611KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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