The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis

doi:10.1007/s11240-012-0264-0

	The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis
	Peng, Xianjun ; Zhang, Lexin ; Zhang, Lixing 1; Liu, Zhujiang 1; Cheng, Liqin ; Yang, Ying 2; Shen, Shihua; Chen, Shuangyan ; Liu, Gongshe
	2013
发表期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
卷号	113 期号:2 页码:245-256
摘要	S-Adenosyl-methionine decarboxylase (SAMDC) and dehydration responsive element-binding proteins (DREBs) can improve plant resistance to abiotic stresses. These proteins have been extensively studied, but the mechanism for transcriptional regulation of SAMDC remains unclear. In this paper, the LcSAMDC2 gene and its promoter were isolated from Leymus chinensis. Two DRE cis-elements were identified from the promoter of LcSAMDC2 and shown to bind with LcDREB2. Subcellular localization and yeast one-hybrid assay revealed that LcDREB2 is a transcription factor. An electrophoretic mobility shift assay (EMSA) showed that LcDREB2 can bind to the LcSAMDC2 promoter probe containing a DRE element. Over-expression of LcDREB2 in L. chinensis callus increased expression of LcSAMDC2. Co-expression of LcDREB2 and the promoter of LcSAMDC2 fused with GUS in tobacco activated GUS activity. These results indicate that LcSAMDC2 is the downstream gene of LcDREB2. In addition, transgenic expression of LcDREB2 and LcSAMDC2 in Arabidopsis can improve the salt stress tolerance of transgenic lines. These results indicate that LcDREB2 cooperating with LcSAMDC2 contributes to resistance to abiotic stress.
关键词	Abiotic stress DREB EMSA Promoter SAMDC
学科领域	Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1007/s11240-012-0264-0
收录类别	SCI
语种	英语
WOS关键词	S-ADENOSYLMETHIONINE DECARBOXYLASE ; POLYAMINES ; STRESS ; GENE ; GROWTH ; OVEREXPRESSION ; IDENTIFICATION ; BIOSYNTHESIS ; ETHYLENE ; LEAVES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000317714300010
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5044
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Basic Research Program of China (973)(National Basic Research Program of China)
引用统计	被引频次：46[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28078
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Chinese Peoples Armed Police Force, Beijing Command Coll, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Peng, Xianjun,Zhang, Lexin,Zhang, Lixing,et al. The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2013,113(2):245-256.
APA	Peng, Xianjun.,Zhang, Lexin.,Zhang, Lixing.,Liu, Zhujiang.,Cheng, Liqin.,...&Liu, Gongshe.(2013).The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis.PLANT CELL TISSUE AND ORGAN CULTURE,113(2),245-256.
MLA	Peng, Xianjun,et al."The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis".PLANT CELL TISSUE AND ORGAN CULTURE 113.2(2013):245-256.

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