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| The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis | |
Peng, Xianjun; Zhang, Lexin; Zhang, Lixing1; Liu, Zhujiang1; Cheng, Liqin; Yang, Ying2; Shen, Shihua ; Chen, Shuangyan; Liu, Gongshe
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| 2013 | |
| Source Publication | PLANT CELL TISSUE AND ORGAN CULTURE
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| ISSN | 0167-6857 |
| Volume | 113Issue:2Pages:245-256 |
| Abstract | S-Adenosyl-methionine decarboxylase (SAMDC) and dehydration responsive element-binding proteins (DREBs) can improve plant resistance to abiotic stresses. These proteins have been extensively studied, but the mechanism for transcriptional regulation of SAMDC remains unclear. In this paper, the LcSAMDC2 gene and its promoter were isolated from Leymus chinensis. Two DRE cis-elements were identified from the promoter of LcSAMDC2 and shown to bind with LcDREB2. Subcellular localization and yeast one-hybrid assay revealed that LcDREB2 is a transcription factor. An electrophoretic mobility shift assay (EMSA) showed that LcDREB2 can bind to the LcSAMDC2 promoter probe containing a DRE element. Over-expression of LcDREB2 in L. chinensis callus increased expression of LcSAMDC2. Co-expression of LcDREB2 and the promoter of LcSAMDC2 fused with GUS in tobacco activated GUS activity. These results indicate that LcSAMDC2 is the downstream gene of LcDREB2. In addition, transgenic expression of LcDREB2 and LcSAMDC2 in Arabidopsis can improve the salt stress tolerance of transgenic lines. These results indicate that LcDREB2 cooperating with LcSAMDC2 contributes to resistance to abiotic stress. |
| Keyword | Abiotic stress DREB EMSA Promoter SAMDC |
| Subject Area | Biotechnology & Applied Microbiology ; Plant Sciences |
| DOI | 10.1007/s11240-012-0264-0 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | S-ADENOSYLMETHIONINE DECARBOXYLASE ; POLYAMINES ; STRESS ; GENE ; GROWTH ; OVEREXPRESSION ; IDENTIFICATION ; BIOSYNTHESIS ; ETHYLENE ; LEAVES |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000317714300010 |
| Publisher | SPRINGER |
| Subtype | Article |
| Publication Place | DORDRECHT |
| EISSN | 1573-5044 |
| Funding Organization | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Basic Research Program of China (973)(National Basic Research Program of China) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/28078 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Chinese Peoples Armed Police Force, Beijing Command Coll, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Peng, Xianjun,Zhang, Lexin,Zhang, Lixing,et al. The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2013,113(2):245-256. |
| APA | Peng, Xianjun.,Zhang, Lexin.,Zhang, Lixing.,Liu, Zhujiang.,Cheng, Liqin.,...&Liu, Gongshe.(2013).The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis.PLANT CELL TISSUE AND ORGAN CULTURE,113(2),245-256. |
| MLA | Peng, Xianjun,et al."The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis".PLANT CELL TISSUE AND ORGAN CULTURE 113.2(2013):245-256. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Peng-2013-The transc（980KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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