Structural and Functional Evolution of Positively Selected Sites in Pine Glutathione S-Transferase Enzyme Family

doi:10.1074/jbc.M113.456863

	Structural and Functional Evolution of Positively Selected Sites in Pine Glutathione S-Transferase Enzyme Family
	Lan, Ting ; Wang, Xiao-Ru ; Zeng, Qing-Yin
	2013
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY
卷号	288 期号:34 页码:24441-24451
摘要	Phylogenetic analyses have identified positive selection as an important driver of protein evolution, both structural and functional. However, the lack of appropriate combined functional and structural assays has generally hindered attempts to elucidate patterns of positively selected sites and their effects on enzyme activity and substrate specificity. In this study we investigated the evolutionary divergence of the glutathione S-transferase (GST) family in Pinus tabuliformis, a pine that is widely distributed from northern to central China, including cold temperate and drought-stressed regions. GSTs play important roles in plant stress tolerance and detoxification. We cloned 44 GST genes from P. tabuliformis and found that 26 of the 44 belong to the largest (Tau) class of GSTs and are differentially expressed across tissues and developmental stages. Substitution models identified five positively selected sites in the Tau GSTs. To examine the functional significance of these positively selected sites, we applied protein structural modeling and site-directed mutagenesis. We found that four of the five positively selected sites significantly affect the enzyme activity and specificity; thus their variation broadens the GST family substrate spectrum. In addition, positive selection has mainly acted on secondary substrate binding sites or sites close to (but not directly at) the primary substrate binding site; thus their variation enables the acquisition of new catalytic functions without compromising the protein primary biochemical properties. Our study sheds light on selective aspects of the functional and structural divergence of the GST family in pine and other organisms.
学科领域	Biochemistry & Molecular Biology
DOI	10.1074/jbc.M113.456863
收录类别	SCI
语种	英语
WOS关键词	GENE FAMILY ; DIVERSIFICATION ; DIVERGENCE ; SYNTHASE ; RESIDUES ; REVEALS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000330612300014
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
文献子类	Article
出版地	BETHESDA
EISSN	1083-351X
资助机构	National Basic Research Program of China(National Basic Research Program of China) ; Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Vetenskapsradet, Sweden(Swedish Research Council)
作者邮箱	Wang, Xiao-Ru/AAC-4444-2020 ; Wang, Xiao-Ru/H-6811-2012
作品OA属性	Green Published, hybrid
引用统计	被引频次：45[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28081
专题	系统与进化植物学国家重点实验室
作者单位	1.[Lan, Ting 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 3.Umea Univ, Dept Ecol & Environm Sci, SE-90187 Umea, Sweden
推荐引用方式 GB/T 7714	Lan, Ting,Wang, Xiao-Ru,Zeng, Qing-Yin. Structural and Functional Evolution of Positively Selected Sites in Pine Glutathione S-Transferase Enzyme Family[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(34):24441-24451.
APA	Lan, Ting,Wang, Xiao-Ru,&Zeng, Qing-Yin.(2013).Structural and Functional Evolution of Positively Selected Sites in Pine Glutathione S-Transferase Enzyme Family.JOURNAL OF BIOLOGICAL CHEMISTRY,288(34),24441-24451.
MLA	Lan, Ting,et al."Structural and Functional Evolution of Positively Selected Sites in Pine Glutathione S-Transferase Enzyme Family".JOURNAL OF BIOLOGICAL CHEMISTRY 288.34(2013):24441-24451.

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