Anthocyanin profile and gene expression in berry skin of two red Vitis vinifera grape cultivars that are sunlight dependent versus sunlight independent

doi:10.1111/ajgw.12023

	Anthocyanin profile and gene expression in berry skin of two red Vitis vinifera grape cultivars that are sunlight dependent versus sunlight independent
	Zheng, Y.; Li, J. H.; Xin, H. P.; Wang, N.; Guan, L.; Wu, B. H.; Li, S. H.
	2013
发表期刊	AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH
ISSN	1322-7130
卷号	19 期号:2 页码:238-248
摘要	Background and Aims When sunlight was excluded (opacity coefficient 100%) from fruitset until maturity, the red grape cultivar Jingxiu failed to colour while Jingyan (Jingxiuxwhite grape Xiangfei) developed red colour. The aim of this study was to gain insight into the genetic background that may be responsible for sunlight-dependent versus sunlight-independent anthocyanin biosynthesis in berry skin. Methods and Results The composition and concentration of anthocyanins via high-performance liquid chromatography, and the transcriptional level of structural and regulatory genes via real-time polymerase chain reaction were investigated. Light exclusion suppressed all the anthocyanins in Jingxiu berry skin, but did not change the proportion of the various anthocyanins in Jingyan. UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) and a v-myb myeloblastosis viral oncogene homolog transcription factor (VvMYBA1) were expressed at a high level in sunlight-exposed Jingxiu, sunlight-exposed and excluded Jingyan, but not in sunlight-excluded Jingxiu, which correlated with phenotypic colouration (red vs not red). There was no difference in the DNA sequence of the VvMYBA1b promoter region between Jingxiu and Jingyan. Conclusions The regulatory gene VvMYBA1 is involved in the regulation of anthocyanin biosynthesis via the structural gene UFGT, while there must be some other regulatory factors or post-transcriptional regulation mechanisms that differentially regulated VvMYBA1 in Jingxiu and Jingyan in response to sunlight. Significance of the Study The primary control mechanism of anthocyanin biosynthesis in the absence of sunlight would provide information for breeding sunlight-independent red grape cultivars, which would be valuable for cultivation in areas with low sunlight, e.g. glasshouses or other covered cropping.
关键词	anthocyanin biosynthesis grape skin colour light exclusion regulatory gene structural gene VvMYBA1 promoter
学科领域	Food Science & Technology ; Horticulture
DOI	10.1111/ajgw.12023
收录类别	SCI
语种	英语
WOS关键词	CABERNET-SAUVIGNON GRAPES ; DIFFERENT LIGHT QUALITIES ; TRANSCRIPTION FACTOR ; VARIETAL DIFFERENCES ; BIOSYNTHETIC GENES ; CHALCONE SYNTHASE ; REGULATORY GENES ; FRUIT ; ACCUMULATION ; EXPOSURE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000319705300012
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1755-0238
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Ministry of Agriculture(Gida Tarim Ve Hayvancilik Bakanligi)
作者邮箱	Wu, Benhong/Q-5328-2016
作品OA属性	gold
引用统计	被引频次：41[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28084
专题	中科院北方资源植物重点实验室
作者单位	1.[Zheng, Y. 2.Li, J. H. 3.Guan, L. 4.Wu, B. H.] Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 5.Guan, L.] Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.[Xin, H. P. 7.Wang, N. 8.Li, S. H.] Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Y.,Li, J. H.,Xin, H. P.,et al. Anthocyanin profile and gene expression in berry skin of two red Vitis vinifera grape cultivars that are sunlight dependent versus sunlight independent[J]. AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH,2013,19(2):238-248.
APA	Zheng, Y..,Li, J. H..,Xin, H. P..,Wang, N..,Guan, L..,...&Li, S. H..(2013).Anthocyanin profile and gene expression in berry skin of two red Vitis vinifera grape cultivars that are sunlight dependent versus sunlight independent.AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH,19(2),238-248.
MLA	Zheng, Y.,et al."Anthocyanin profile and gene expression in berry skin of two red Vitis vinifera grape cultivars that are sunlight dependent versus sunlight independent".AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH 19.2(2013):238-248.

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