Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation

doi:10.1016/j.jprot.2013.06.030

	Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation
	Liu, Hui 1; Wang, Cuiping 1; Komatsu, Setsuko 2; He, Mingxia 3; Liu, Gongshe; Shen, Shihua
	2013
发表期刊	JOURNAL OF PROTEOMICS
ISSN	1874-3919
卷号	91 页码:23-40
摘要	To characterize the metabolic signatures of lipid accumulation in Jatropha curcas seeds, comparative proteomic technique was employed to Profile protein changes during the seed development. Temporal changes in comparative proteome were examined using gels-based proteomic technique at six developmental stages for lipid accumulation. And 104 differentially expressed proteins were identified by MALDI-TOF/TOF tandem mass spectrometry. These protein species were classified into 10 functional categories, and the results demonstrated that protein species related to energy and metabolism were notably accumulated and involved in the carbon flux to lipid accumulation that occurs primarily from early to late stage in seed development. Glycolysis and oxidative pentose phosphate pathways were the major pathways of producing carbon flux, and the glucose-6-phosphate and triose-phosphate are the major carbon source for fatty acid synthesis. Lipid analysis revealed that fatty acid accumulation initiated 25 days after flowering at the late stage of seed development of J. curcas. Furthermore, C-16:0 was initially synthesized as the precursor for the elongation to C-18:1 and C-18:2 in the developing seeds of J. curcas. Together, the metabolic signatures on protein changes in seed development provide profound knowledge and perspective insights into understanding lipid network in J. curcas. Biological significance Due to the abundant oil content in seeds, Jatropha curcas seeds are being considered as the ideal materials for biodiesel. Although several studies had carried out the transcriptomic project to study the genes expression profiles in seed development of J. curcas, these ESTs hadn't been confirmed by qRT-PCR. Yet, the seed development of J. curcas had been described for a pool of developing seeds instead of being characterized systematically. Moreover, cellular metabolic events are also controlled by protein protein interactions, posttranslational protein modifications, and enzymatic activities which cannot be described by transcriptional profiling approaches alone. In this study, within the overall objective of profiling differential protein abundance in developing J. curcas seeds, we provide a setting of physiological data with dynamic proteomic and qRT-PCR analysis to characterize the metabolic pathways and the relationship between mRNA and protein patterns from early stage to seed filling during the seed development of J. curcas. The construction of J. curcas seed development proteome profiles will significantly increase our understanding of the process of seed development and provide a foundation to examine the dynamic changes of the metabolic network during seed development process and certainly suggest some clues to improve the lipid content off. curcas seeds. (C) 2013 Elsevier B.V. All rights reserved.
关键词	Jatropha curcas Proteomics Seed development Carbon flux Lipid accumulation
学科领域	Biochemical Research Methods
DOI	10.1016/j.jprot.2013.06.030
收录类别	SCI
语种	英语
WOS关键词	CARRIER-PROTEIN DESATURASE ; FATTY-ACID SYNTHESIS ; ACETYL-COENZYME-A ; TRANSCRIPTOME ANALYSIS ; BRASSICA-NAPUS ; SYNTHASE-III ; OIL ; PLANT ; EXPRESSION ; BIODIESEL
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000327906000003
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1876-7737
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences)
作者邮箱	Komatsu, Setsuko/HNI-6864-2023
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28086
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Res & Dev Resource Plant, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan 4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,et al. Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation[J]. JOURNAL OF PROTEOMICS,2013,91:23-40.
APA	Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,He, Mingxia,Liu, Gongshe,&Shen, Shihua.(2013).Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation.JOURNAL OF PROTEOMICS,91,23-40.
MLA	Liu, Hui,et al."Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation".JOURNAL OF PROTEOMICS 91(2013):23-40.

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