Embryo-specific expression of soybean oleosin altered oil body morphogenesis and increased lipid content in transgenic rice seeds

doi:10.1007/s00122-013-2135-4

	Embryo-specific expression of soybean oleosin altered oil body morphogenesis and increased lipid content in transgenic rice seeds
	Liu, Wen Xian 1; Liu, Hua Liang ; Qu, Le Qing
	2013
发表期刊	THEORETICAL AND APPLIED GENETICS
ISSN	0040-5752
卷号	126 期号:9 页码:2289-2297
摘要	Oleosin is the most abundant protein in the oil bodies of plant seeds, playing an important role in regulating oil body formation and lipid accumulation. To investigate whether lipid accumulation in transgenic rice seeds depends on the expression level of oleosin, we introduced two soybean oleosin genes encoding 24 kDa proteins into rice under the control of an embryo-specific rice promoter REG-2. Overexpression of soybean oleosin in transgenic rice leads to an increase of seed lipid content up to 36.93 and 46.06 % higher than that of the non-transgenic control, respectively, while the overall fatty acid profiles of triacylglycerols remained unchanged. The overexpression of soybean oleosin in transgenic rice seeds resulted in more numerous and smaller oil bodies compared with wild type, suggesting that an inverse relationship exists between oil body size and the total oleosin level. The increase in lipid content is accompanied by a reduction in the accumulation of total seed protein. Our results suggest that it is possible to increase rice seed oil content for food use and for use as a low-cost feedstock for biodiesel by overexpressing oleosin in rice seeds.
学科领域	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
DOI	10.1007/s00122-013-2135-4
收录类别	SCI
语种	英语
WOS关键词	ARABIDOPSIS-THALIANA ; GENE PROMOTERS ; BRAN OIL ; BODIES ; ACCUMULATION ; ISOFORMS ; ACID ; PROTEIN ; BIODIESEL ; OILBODIES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000323671100008
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
资助机构	National Program of Transgenic Variety Development of China ; National High Technology Research and Development Program (863)(National High Technology Research and Development Program of China) ; Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
引用统计	被引频次：41[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28091
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wen Xian,Liu, Hua Liang,Qu, Le Qing. Embryo-specific expression of soybean oleosin altered oil body morphogenesis and increased lipid content in transgenic rice seeds[J]. THEORETICAL AND APPLIED GENETICS,2013,126(9):2289-2297.
APA	Liu, Wen Xian,Liu, Hua Liang,&Qu, Le Qing.(2013).Embryo-specific expression of soybean oleosin altered oil body morphogenesis and increased lipid content in transgenic rice seeds.THEORETICAL AND APPLIED GENETICS,126(9),2289-2297.
MLA	Liu, Wen Xian,et al."Embryo-specific expression of soybean oleosin altered oil body morphogenesis and increased lipid content in transgenic rice seeds".THEORETICAL AND APPLIED GENETICS 126.9(2013):2289-2297.

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