Comparative Proteomics of Thellungiella halophila Leaves from Plants Subjected to Salinity Reveals the Importance of Chloroplastic Starch and Soluble Sugars in Halophyte Salt Tolerance

doi:10.1074/mcp.M112.022475

IB-CAS > 中科院光生物学重点实验室

	Comparative Proteomics of Thellungiella halophila Leaves from Plants Subjected to Salinity Reveals the Importance of Chloroplastic Starch and Soluble Sugars in Halophyte Salt Tolerance
	Wang, Xuchu 1; Chang, Lili 1; Wang, Baichen 2; Wang, Dan ; Li, Pinghua 1; Wang, Limin 1; Yi, Xiaoping ; Huang, Qixing ; Peng, Ming ; Guo, Anping 1
	2013
发表期刊	MOLECULAR & CELLULAR PROTEOMICS
ISSN	1535-9476
卷号	12 期号:8 页码:2174-2195
摘要	Thellungiella halophila, a close relative of Arabidopsis, is a model halophyte used to study plant salt tolerance. The proteomic/physiological/transcriptomic analyses of Thellungiella plant leaves subjected to different salinity levels, reported herein, indicate an extraordinary ability of Thellungiella to adapt to large concentrations of exogenous saline by compartmentalizing Na+ into cell vacuoles and accumulating proline and soluble sugars as organic osmolytes. Salinity stress stimulated the accumulation of starch in chloroplasts, which resulted in a greatly increased content of starch and total sugars in leaves. Comparative proteomics of Thellungiella leaves identified 209 salt-responsive proteins. Among these, the sequences of 108 proteins were strongly homologous to Arabidopsis protein sequences, and 30 had previously been identified as Thellungiella proteins. Functional classification of these proteins into 16 categories indicated that the majority are involved in carbohydrate metabolism, followed by those involved in energy production and conversion, and then those involved in the transport of inorganic ions. Pathway analysis revealed that most of the proteins are involved in starch and sucrose metabolism, carbon fixation, photosynthesis, and glycolysis. Of these processes, the most affected were starch and sucrose metabolism, which might be pivotal for salt tolerance. The gene expression patterns of the 209 salt-responsive proteins revealed through hierarchical clustering of microarray data and the expression patterns of 29 Thellungiella genes evaluated via quantitative RT-PCR were similar to those deduced via proteomic analysis, which underscored the possibility that starch and sucrose metabolism might play pivotal roles in determining the salt tolerance ability of Thellungiella. Our observations enabled us to propose a schematic representation of the systematic salt-tolerance phenotype in Thellungiella and suggested that the increased accumulation of starch, soluble sugars, and proline, as well as subcellular compartmentalization of sodium, might collectively denote important mechanisms for halophyte salt tolerance.
学科领域	Biochemical Research Methods
DOI	10.1074/mcp.M112.022475
收录类别	SCI
语种	英语
WOS关键词	ARABIDOPSIS-THALIANA ; GENE-EXPRESSION ; NA+ TRANSPORT ; STRESS ; GROWTH ; ACCUMULATION ; PROLINE ; MODEL ; NACL ; OVEREXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000328839100012
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
文献子类	Article
出版地	BETHESDA
EISSN	1535-9484
资助机构	National Basic Research Program (973) of China(National Basic Research Program of China) ; National Natural Science Foundation(National Natural Science Foundation of China (NSFC)) ; Program for the Top Young Talents in Chinese Academy of Tropical Agricultural Sciences
作者邮箱	Li, Ping/GYV-4914-2022 ; Wang, Xuchu/E-7133-2016
作品OA属性	hybrid, Green Published, Green Accepted
引用统计	被引频次：115[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28097
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Trop Agr Sci, Key Lab Biol & Genet Resources Trop Crops, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China 2.Hainan Univ, Haikou 570228, Hainan, Peoples R China 3.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xuchu,Chang, Lili,Wang, Baichen,et al. Comparative Proteomics of Thellungiella halophila Leaves from Plants Subjected to Salinity Reveals the Importance of Chloroplastic Starch and Soluble Sugars in Halophyte Salt Tolerance[J]. MOLECULAR & CELLULAR PROTEOMICS,2013,12(8):2174-2195.
APA	Wang, Xuchu.,Chang, Lili.,Wang, Baichen.,Wang, Dan.,Li, Pinghua.,...&Guo, Anping.(2013).Comparative Proteomics of Thellungiella halophila Leaves from Plants Subjected to Salinity Reveals the Importance of Chloroplastic Starch and Soluble Sugars in Halophyte Salt Tolerance.MOLECULAR & CELLULAR PROTEOMICS,12(8),2174-2195.
MLA	Wang, Xuchu,et al."Comparative Proteomics of Thellungiella halophila Leaves from Plants Subjected to Salinity Reveals the Importance of Chloroplastic Starch and Soluble Sugars in Halophyte Salt Tolerance".MOLECULAR & CELLULAR PROTEOMICS 12.8(2013):2174-2195.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
23660471.pdf（7649KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文