The euAP1 Protein MPF3 Represses MPF2 to Specify Floral Calyx Identity and Displays Crucial Roles in Chinese Lantern Development in Physalis

doi:10.1105/tpc.113.111757

	The euAP1 Protein MPF3 Represses MPF2 to Specify Floral Calyx Identity and Displays Crucial Roles in Chinese Lantern Development in Physalis
	Zhao, Jing ; Tian, Ying ; Zhang, Ji-Si ; Zhao, Man 1; Gong, Pichang 1; Riss, Simone 2; Saedler, Rainer 2; He, Chaoying
	2013
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	25 期号:6 页码:2002-2021
摘要	The Chinese lantern phenotype or inflated calyx syndrome (ICS) is a postfloral morphological novelty in Physalis. Its origin is associated with the heterotopic expression of the MADS box gene 2 from Physalis floridana (MPF2) in floral organs, yet the process underlying its identity remains elusive. Here, we show that MPF3, which is expressed specifically in floral tissues, encodes a core eudicot APETALA1-like (euAP1) MADS-domain protein. MPF3 was primarily localized to the nucleus, and it interacted with MPF2 and some floral MADS-domain proteins to selectively bind the CC-A-rich-GG (CArG) boxes in the MPF2 promoter. Downregulating MPF3 resulted in a dramatic elevation in MPF2 in the calyces and androecium, leading to enlarged and leaf-like floral calyces; however, the postfloral lantern was smaller and deformed. Starch accumulation in pollen was blocked. MPF3 MPF2 double knockdowns showed normal floral calyces and more mature pollen than those found in plants in which either MPF3 or MPF2 was downregulated. Therefore, MPF3 specifies calyx identity and regulates ICS formation and male fertility through interactions with MPF2/MPF2. Furthermore, both genes were found to activate Physalis floridana invertase gene 4 homolog, which encodes an invertase cleaving Suc, a putative key gene in sugar partitioning. The novel role of the MPF3-MPF2 regulatory circuit in male fertility is integral to the origin of ICS. Our results shed light on the evolution and development of ICS in Physalis and on the functional evolution of euAP1s in angiosperms.
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1105/tpc.113.111757
收录类别	SCI
语种	英语
WOS关键词	MADS-DOMAIN PROTEINS ; DETECTING POSITIVE SELECTION ; HOMEOTIC GENE ; BOX GENE ; HETEROTOPIC EXPRESSION ; MORPHOLOGICAL NOVELTY ; TRANSCRIPTION FACTORS ; ARABIDOPSIS-THALIANA ; FLOWER DEVELOPMENT ; MERISTEM IDENTITY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000322371500012
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-298X
资助机构	Chinese Academy of Sciences(Chinese Academy of Sciences) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; 46th Postdoctoral Science Foundation of China
作者邮箱	Gong, Pichang/GVT-9910-2022 ; zhao, jing/B-3868-2019
作品OA属性	Green Published
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28101
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Max Planck Inst Plant Breeding Res, Dept Mol Plant Genet, D-50829 Cologne, Germany
推荐引用方式 GB/T 7714	Zhao, Jing,Tian, Ying,Zhang, Ji-Si,et al. The euAP1 Protein MPF3 Represses MPF2 to Specify Floral Calyx Identity and Displays Crucial Roles in Chinese Lantern Development in Physalis[J]. PLANT CELL,2013,25(6):2002-2021.
APA	Zhao, Jing.,Tian, Ying.,Zhang, Ji-Si.,Zhao, Man.,Gong, Pichang.,...&He, Chaoying.(2013).The euAP1 Protein MPF3 Represses MPF2 to Specify Floral Calyx Identity and Displays Crucial Roles in Chinese Lantern Development in Physalis.PLANT CELL,25(6),2002-2021.
MLA	Zhao, Jing,et al."The euAP1 Protein MPF3 Represses MPF2 to Specify Floral Calyx Identity and Displays Crucial Roles in Chinese Lantern Development in Physalis".PLANT CELL 25.6(2013):2002-2021.

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