Insights into enzyme secretion by filamentous fungi: Comparative proteome analysis of Trichoderma reesei grown on different carbon sources

doi:10.1016/j.jprot.2013.06.014

IB-CAS > 中科院光生物学重点实验室

	Insights into enzyme secretion by filamentous fungi: Comparative proteome analysis of Trichoderma reesei grown on different carbon sources
	He Jun ; Han Guangye 1; Chen Daiwen
	2013
发表期刊	JOURNAL OF PROTEOMICS
ISSN	1874-3919
卷号	89 页码:191-201
摘要	Trichoderma reesei is the main industrial producer of lignocellulolytic enzymes, and the secretory behavior of this fungus strongly depends on the carbon sources. To gain insights into how the T. reesei adapts to various carbons and regulates enzyme production, the extra-and intracellular proteomes of T. reesei grown in defined medium with lactose or xylose as the carbon source were investigated. Results indicated that the composition of extracellular proteome differed considerably depending on the carbons. The main cellobiohydrolases, i.e. Cel7a/Cel6 were the most abundant cellulolytic enzymes identified in both media, and found to be more abundant in lactose-grown culture. As compared to lactose, xylose can serve as a potent inducer of xylanolytic enzymes. Interestingly, most identified intracellular proteins are involved in carbon metabolism. Enzymes involved in utilization of xylose, such as n-xylose reductase (Xyl1p) and n-xylose dehydrogenase (Xyl2p), were present at elevated levels in the culture growing on xylose but only in minor amounts in the lactose culture. However, lactose induction significantly activated the expression of key enzymes involved in glycolysis pathway and citrate cycle. Importantly, the protein Xyl1p which participates both in the lactose and the xylose catabolism was verified as a potential regulator for cellulase formation in T. reesei. Biological significance This study not only gives an overview of the ubiquitous cellular changes induced by the two conventional carbon substrates, but offers the framework for understanding the mechanisms behind the carbon-dependent induction of extracellular enzymes in T. reesei. Moreover, this study provides a potential target (Xyl1p) that could be tentatively used for metabolic engineering of T. reesei for cost-effective cellulase production. (C) 2013 Elsevier B.V. All rights reserved.
关键词	Trichoderma reesei Proteome Carbon source Enzyme Regulation
学科领域	Biochemical Research Methods
DOI	10.1016/j.jprot.2013.06.014
收录类别	SCI
语种	英语
WOS关键词	CELLULASE GENE-EXPRESSION ; HYPOCREA-JECORINA ; SACCHAROMYCES-CEREVISIAE ; BETA-GALACTOSIDASE ; ASPERGILLUS-NIGER ; INDUCTION ; XYLOSE ; XYLANASE ; HEMICELLULASES ; TRANSFORMATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000323867200016
出版者	ELSEVIER SCIENCE BV
文献子类	Article
出版地	AMSTERDAM
EISSN	1876-7737
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Special Fund of Chongqing Key Laboratory (CSTC)(Natural Science Foundation Project of CQ CSTC)
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28108
专题	中科院光生物学重点实验室
作者单位	1.Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Sichuan, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	He Jun,Han Guangye,Chen Daiwen. Insights into enzyme secretion by filamentous fungi: Comparative proteome analysis of Trichoderma reesei grown on different carbon sources[J]. JOURNAL OF PROTEOMICS,2013,89:191-201.
APA	He Jun,Han Guangye,&Chen Daiwen.(2013).Insights into enzyme secretion by filamentous fungi: Comparative proteome analysis of Trichoderma reesei grown on different carbon sources.JOURNAL OF PROTEOMICS,89,191-201.
MLA	He Jun,et al."Insights into enzyme secretion by filamentous fungi: Comparative proteome analysis of Trichoderma reesei grown on different carbon sources".JOURNAL OF PROTEOMICS 89(2013):191-201.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
23796490.pdf（1012KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文