Optimized Agroinfiltration and Virus-Induced Gene Silencing to Study Ve1-Mediated Verticillium Resistance in Tobacco

doi:10.1094/MPMI-06-12-0161-R

	Optimized Agroinfiltration and Virus-Induced Gene Silencing to Study Ve1-Mediated Verticillium Resistance in Tobacco
	Zhang, Zhao ; Fradin, Emilie ; de Jonge, Ronnie ; van Esse, H. Peter ; Smit, Patrick ; Liu, Chun-Ming; Thomma, Bart P. H. J.
	2013
发表期刊	MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN	0894-0282
卷号	26 期号:2 页码:182-190
摘要	Recognition of pathogen effectors by plant immune receptors often leads to the activation of a hypersensitive response (HR), which is a rapid and localized cell death of plant tissue surrounding the site at which recognition occurs. Due to its particular amenability to transient assays for functional genetics, tobacco is a model for immune signaling in the Solanaceae plant family. Here, we show that coexpression of the tomato (Solanum lycopersicum) immune receptor Ve1 and the corresponding Verticillium effector protein Ave1 leads to HR only in particular tobacco species. Whereas HR is obtained in Nicotiana tabacum, no such response is obtained in N. benthamiana. Furthermore, our analysis revealed an endogenous Ve1 ortholog in Nicotiana glutinosa, as expression of Ave1 in absence of Ve1 induced a HR, and N. glutinosa was found to be resistant against race 1 Verticillium dahliae. We furthermore report the establishment of virus-induced gene silencing in N. tabacum for functional analysis of Ve1 signaling. Collectively, our data show that N. tabacum can be used as a model plant to study Ve1-mediated immune signaling.
学科领域	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1094/MPMI-06-12-0161-R
收录类别	SCI
语种	英语
WOS关键词	RECEPTOR-LIKE PROTEINS ; TRANSIENT EXPRESSION SYSTEM ; FUNCTIONAL-ANALYSIS ; DISEASE RESISTANCE ; NICOTIANA-BENTHAMIANA ; PLANT-PATHOGEN ; BINARY VECTORS ; ALBO-ATRUM ; TOMATO ; VERSATILE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000314003000004
出版者	AMER PHYTOPATHOLOGICAL SOC
文献子类	Article
出版地	ST PAUL
EISSN	1943-7706
资助机构	Netherlands Organization for Scientific Research (NWO-ALW)(Netherlands Organization for Scientific Research (NWO)) ; Wageningen University ; Centre for BioSystems Genomics (CBSG) ; ERA-NET Plant Genomics
作者邮箱	Thomma, Bart/ABH-6344-2020 ; Liu, Chun-Ming/G-1006-2010 ; Thomma, Bart/E-5959-2011 ; Zhang, Zhao/J-1615-2019 ; de Jonge, Ronnie/I-3260-2016 ; 刘, 春明/GLU-4320-2022 ; van Esse, Peter/U-5304-2018
作品OA属性	hybrid
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28117
专题	中科院植物分子生理学重点实验室
作者单位	1.[Zhang, Zhao 2.Fradin, Emilie 3.de Jonge, Ronnie 4.van Esse, H. Peter 5.Smit, Patrick 6.Thomma, Bart P. H. J.] Wageningen Univ, Phytopathol Lab, NL-6708 PB Wageningen, Netherlands 7.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 8.[Fradin, Emilie 9.Thomma, Bart P. H. J.] Ctr BioSyst Genom, NL-6700 AB Wageningen, Netherlands
推荐引用方式 GB/T 7714	Zhang, Zhao,Fradin, Emilie,de Jonge, Ronnie,et al. Optimized Agroinfiltration and Virus-Induced Gene Silencing to Study Ve1-Mediated Verticillium Resistance in Tobacco[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2013,26(2):182-190.
APA	Zhang, Zhao.,Fradin, Emilie.,de Jonge, Ronnie.,van Esse, H. Peter.,Smit, Patrick.,...&Thomma, Bart P. H. J..(2013).Optimized Agroinfiltration and Virus-Induced Gene Silencing to Study Ve1-Mediated Verticillium Resistance in Tobacco.MOLECULAR PLANT-MICROBE INTERACTIONS,26(2),182-190.
MLA	Zhang, Zhao,et al."Optimized Agroinfiltration and Virus-Induced Gene Silencing to Study Ve1-Mediated Verticillium Resistance in Tobacco".MOLECULAR PLANT-MICROBE INTERACTIONS 26.2(2013):182-190.

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