Arabidopsis Villins Promote Actin Turnover at Pollen Tube Tips and Facilitate the Construction of Actin Collars

doi:10.1105/tpc.113.110940

	Arabidopsis Villins Promote Actin Turnover at Pollen Tube Tips and Facilitate the Construction of Actin Collars
	Qu, Xiaolu ; Zhang, Hua ; Xie, Yurong ; Wang, Juan ; Chen, Naizhi ; Huang, Shanjin 1
	2013
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	25 期号:5 页码:1803-1817
摘要	Apical actin filaments are crucial for pollen tube tip growth. However, the specific dynamic changes and regulatory mechanisms associated with actin filaments in the apical region remain largely unknown. Here, we have investigated the quantitative dynamic parameters that underlie actin filament growth and disappearance in the apical regions of pollen tubes and identified villin as the major player that drives rapid turnover of actin filaments in this region. Downregulation of Arabidopsis thaliana VILLIN2 (VLN2) and VLN5 led to accumulation of actin filaments at the pollen tube apex. Careful analysis of single filament dynamics showed that the severing frequency significantly decreased, and the lifetime significantly increased in vln2 vln5 pollen tubes. These results indicate that villin-mediated severing is critical for turnover and departure of actin filaments originating in the apical region. Consequently, the construction of actin collars was affected in vln2 vln5 pollen tubes. In addition to the decrease in severing frequency, actin filaments also became wavy and buckled in the apical cytoplasm of vln2 vln5 pollen tubes. These results suggest that villin confers rigidity upon actin filaments. Furthermore, an observed decrease in skewness of actin filaments in the subapical region of vln2 vln5 pollen tubes suggests that villin-mediated bundling activity may also play a role in the construction of actin collars. Thus, our data suggest that villins promote actin turnover at pollen tube tips and facilitate the construction of actin collars.
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1105/tpc.113.110940
收录类别	SCI
语种	英语
WOS关键词	F-ACTIN ; PHYSCOMITRELLA-PATENS ; DEPOLYMERIZING FACTOR ; POLARIZED GROWTH ; CELL-GROWTH ; EXTRACELLULAR CALCIUM ; INTERACTING PROTEIN1 ; DYNAMIC-BEHAVIOR ; BUNDLING PROTEIN ; CORTICAL ARRAY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000321035800025
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
资助机构	Ministry of Science and Technology of China(Ministry of Science and Technology, China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
作者邮箱	Huang, Shanjin/M-4112-2017 ; Wang, Juan/AAH-5872-2020
作品OA属性	Bronze, Green Accepted
引用统计	被引频次：83[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28118
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Qu, Xiaolu,Zhang, Hua,Xie, Yurong,et al. Arabidopsis Villins Promote Actin Turnover at Pollen Tube Tips and Facilitate the Construction of Actin Collars[J]. PLANT CELL,2013,25(5):1803-1817.
APA	Qu, Xiaolu,Zhang, Hua,Xie, Yurong,Wang, Juan,Chen, Naizhi,&Huang, Shanjin.(2013).Arabidopsis Villins Promote Actin Turnover at Pollen Tube Tips and Facilitate the Construction of Actin Collars.PLANT CELL,25(5),1803-1817.
MLA	Qu, Xiaolu,et al."Arabidopsis Villins Promote Actin Turnover at Pollen Tube Tips and Facilitate the Construction of Actin Collars".PLANT CELL 25.5(2013):1803-1817.

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