The F-Box Protein OsFBK12 Targets OsSAMS1 for Degradation and Affects Pleiotropic Phenotypes, Including Leaf Senescence, in Rice

doi:10.1104/pp.113.224527

	The F-Box Protein OsFBK12 Targets OsSAMS1 for Degradation and Affects Pleiotropic Phenotypes, Including Leaf Senescence, in Rice
	Chen, Yuan 1; Xu, Yunyuan ; Luo, Wei ; Li, Wenxuan ; Chen, Na ; Zhang, Dajian 1; Chong, Kang 2
	2013
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	163 期号:4 页码:1673-1685
摘要	Leaf senescence is related to the grain-filling rate and grain weight in cereals. Many components involved in senescence regulation at either the genetic or physiological level are known. However, less is known about molecular regulation mechanisms. Here, we report that OsFBK12 (an F-box protein containing a Kelch repeat motif) interacts with S-ADENOSYL-L-METHIONINE SYNTHETASE1 (SAMS1) to regulate leaf senescence and seed size as well as grain number in rice (Oryza sativa). Yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays indicate that OsFBK12 interacts with Oryza sativa S-PHASE KINASE-ASSOCIATED PROTEIN1-LIKE PROTEIN and with OsSAMS1. Biochemical and physiological data showed that OsFBK12 targets OsSAMS1 for degradation. OsFBK12-RNA interference lines and OsSAMS1 overexpression lines showed increased ethylene levels, while OsFBK12-OX lines and OsSAMS1-RNA interference plants exhibited decreased ethylene. Phenotypically, overexpression of OsFBK12 led to a delay in leaf senescence and germination and increased seed size, whereas knockdown lines of either OsFBK12 or OsSAMS1 promoted the senescence program. Our results suggest that OsFBK12 is involved in the 26S proteasome pathway by interacting with Oryza sativa S-PHASE KINASE-ASSOCIATED PROTEIN1-LIKE PROTEIN and that it targets the substrate OsSAMS1 for degradation, triggering changes in ethylene levels for the regulation of leaf senescence and grain size. These data have potential applications in the molecular breeding of rice.
学科领域	Plant Sciences
DOI	10.1104/pp.113.224527
收录类别	SCI
语种	英语
WOS关键词	ADENOSYL-L-METHIONINE ; GENOME-WIDE ANALYSIS ; SEED DEVELOPMENT ; ETHYLENE ; GENE ; METHYLATION ; OVEREXPRESSION ; POLYAMINES ; EXPRESSION ; MUTATIONS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000327942800015
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	Major State Basic Research Program 973(National Basic Research Program of China) ; National Natural Sciences Foundation of China(National Natural Science Foundation of China (NSFC))
作品OA属性	Green Accepted
引用统计	被引频次：100[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28125
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Plant Gene Res Ctr, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yuan,Xu, Yunyuan,Luo, Wei,et al. The F-Box Protein OsFBK12 Targets OsSAMS1 for Degradation and Affects Pleiotropic Phenotypes, Including Leaf Senescence, in Rice[J]. PLANT PHYSIOLOGY,2013,163(4):1673-1685.
APA	Chen, Yuan.,Xu, Yunyuan.,Luo, Wei.,Li, Wenxuan.,Chen, Na.,...&Chong, Kang.(2013).The F-Box Protein OsFBK12 Targets OsSAMS1 for Degradation and Affects Pleiotropic Phenotypes, Including Leaf Senescence, in Rice.PLANT PHYSIOLOGY,163(4),1673-1685.
MLA	Chen, Yuan,et al."The F-Box Protein OsFBK12 Targets OsSAMS1 for Degradation and Affects Pleiotropic Phenotypes, Including Leaf Senescence, in Rice".PLANT PHYSIOLOGY 163.4(2013):1673-1685.

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