The ER luminal binding protein (BiP) alleviates Cd2+-induced programmed cell death through endoplasmic reticulum stress-cell death signaling pathway in tobacco cells

doi:10.1016/j.jplph.2013.05.017

	The ER luminal binding protein (BiP) alleviates Cd2+-induced programmed cell death through endoplasmic reticulum stress-cell death signaling pathway in tobacco cells
	Xu, Hua 1; Xu, Wenzhong ; Xi, Hongmei 1; Ma, Wenwen ; He, Zhenyan; Ma, Mi
	2013
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
卷号	170 期号:16 页码:1434-1441
摘要	Cadmium (Cd) is very toxic to plant cells and Cd2+ stress induces programmed cell death (PCD) in Nicotiana tabacum L cv. bright yellow-2 (BY-2) cells. In plants, PCD can be regulated through the endoplasmic reticulum (ER) stress-cell death signaling pathway. However, the mechanism of Cd2+-induced PCD remains unclear. In this study, we found that Cd2+ treatment induced ER stress in tobacco BY-2 cells. The expression of two ER stress markers NtBLP4 and NtPDI and an unfolded protein response related transcription factor NtbZIP60 were upregulated with Cd2+ stress. Meanwhile, the PCD triggered by prolonged Cd2+ stress could be relieved by two ER chemical chaperones, 4-phenylbutyric acid and tauroursodeoxycholic acid. These results demonstrate that the ER stress-cell death signaling pathway participates in the mediation of Cd2+-induced PCD. Furthermore, the ER chaperone AtBiP2 protein alleviated Cd2+-induced ER stress and PCD in BY-2 cells based on the fact that heterologous expression of AtBiP2 in tobacco BY-2 cells reduced the expression of NtBLP4 and a PCD-related gene NtHsr203J under Cd2+ stress conditions. In summary, these results suggest that the ER stress-cell death signaling pathway regulates Cd2+-induced PCD in tobacco BY-2 cells, and that the AtBiP2 protein act as a negative regulator in this process. (C) 2013 Elsevier GmbH. All rights reserved.
关键词	BiP protein Bright yellow-2 cells Cadmium ER stress Programmed cell death
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2013.05.017
收录类别	SCI
语种	英语
WOS关键词	BZIP TRANSCRIPTION FACTOR ; CADMIUM TOXICITY ; CHAPERONE PROTEIN ; ARABIDOPSIS ; APOPTOSIS ; INDUCTION ; IRE1 ; IDENTIFICATION ; ACCUMULATION ; TRANSLATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000325301500006
出版者	ELSEVIER GMBH
文献子类	Article
出版地	MUNICH
EISSN	1618-1328
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
引用统计	被引频次：50[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28139
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Hua,Xu, Wenzhong,Xi, Hongmei,et al. The ER luminal binding protein (BiP) alleviates Cd2+-induced programmed cell death through endoplasmic reticulum stress-cell death signaling pathway in tobacco cells[J]. JOURNAL OF PLANT PHYSIOLOGY,2013,170(16):1434-1441.
APA	Xu, Hua,Xu, Wenzhong,Xi, Hongmei,Ma, Wenwen,He, Zhenyan,&Ma, Mi.(2013).The ER luminal binding protein (BiP) alleviates Cd2+-induced programmed cell death through endoplasmic reticulum stress-cell death signaling pathway in tobacco cells.JOURNAL OF PLANT PHYSIOLOGY,170(16),1434-1441.
MLA	Xu, Hua,et al."The ER luminal binding protein (BiP) alleviates Cd2+-induced programmed cell death through endoplasmic reticulum stress-cell death signaling pathway in tobacco cells".JOURNAL OF PLANT PHYSIOLOGY 170.16(2013):1434-1441.

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