A Simple CELI Endonuclease-Based Protocol for Genotyping both SNPs and InDels

doi:10.1007/s11105-013-0606-z

	A Simple CELI Endonuclease-Based Protocol for Genotyping both SNPs and InDels
	Jiang, Guo-Qiang ; Yao, Xue-Feng ; Liu, Chun-Ming
	2013
发表期刊	PLANT MOLECULAR BIOLOGY REPORTER
ISSN	0735-9640
卷号	31 期号:6 页码:1325-1335
摘要	Modern genetic analyses rely on efficient genotyping of single-nucleotide polymorphisms (SNP) or insertion/deletion length polymorphisms (InDel) in genomes. Methods available to genotype these polymorphisms include sequencing, cleaved amplified polymorphic sequence, high-resolution DNA melting, and microarray analyses, which are all rather tedious or expensive to set up for daily use. Here, we report a simplified label-free CELI endonuclease (CELI)-based protocol that enables us to detect both SNPs and InDels for fragment lengths between 500 and 6 kb. PCR-amplified target DNA fragments were annealed, cleaved by CELI, and analyzed either cost-effectively by agarose gel electrophoresis or automatically by capillary electrophoresis. The optimal amplification sizes, potential blind ends, and the maximum pooling capacities were examined for both electrophoresis protocols. We believe that the CELI-based genotyping protocol can be used in the detection of mutations, marker-assisted breeding, map-based cloning, and genome-wide association studies.
关键词	CELI SNP InDel Agarose gel electrophoresis Capillary electrophoresis
学科领域	Biochemical Research Methods ; Plant Sciences
DOI	10.1007/s11105-013-0606-z
收录类别	SCI
语种	英语
WOS关键词	INDUCED MUTATIONS ; MELTING ANALYSIS ; RICE ; DISCOVERY ; POLYMORPHISMS ; SEQUENCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000326804400014
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1572-9818
资助机构	National Transgenic Research Projects ; EU CEDROME project
作者邮箱	刘, 春明/GLU-4320-2022 ; Liu, Chun-Ming/G-1006-2010 ; Yao, XF/AAM-5959-2020
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28148
专题	中科院植物分子生理学重点实验室
作者单位	1.[Jiang, Guo-Qiang 2.Yao, Xue-Feng 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Guo-Qiang,Yao, Xue-Feng,Liu, Chun-Ming. A Simple CELI Endonuclease-Based Protocol for Genotyping both SNPs and InDels[J]. PLANT MOLECULAR BIOLOGY REPORTER,2013,31(6):1325-1335.
APA	Jiang, Guo-Qiang,Yao, Xue-Feng,&Liu, Chun-Ming.(2013).A Simple CELI Endonuclease-Based Protocol for Genotyping both SNPs and InDels.PLANT MOLECULAR BIOLOGY REPORTER,31(6),1325-1335.
MLA	Jiang, Guo-Qiang,et al."A Simple CELI Endonuclease-Based Protocol for Genotyping both SNPs and InDels".PLANT MOLECULAR BIOLOGY REPORTER 31.6(2013):1325-1335.

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