Knowledge Management System Of Institute Of Botany,CAS
| Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization | |
| Wang, Qinli; Zhao, Yuanyuan; Luo, Wangxi1; Li, Ruili; He, Qihua2; Fang, Xiaohong1; De Michele, Roberto3,4; Ast, Cindy3; von Wiren, Nicolaus5; Lin, Jinxing | |
| 2013 | |
| 发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| ISSN | 0027-8424 |
| 卷号 | 110期号:32页码:13204-13209 |
| 摘要 | Ammonium is a preferred source of nitrogen for plants but is toxic at high levels. Plant ammonium transporters (AMTs) play an essential role in NH4+ uptake, but the mechanism by which AMTs are regulated remains unclear. To study how AMTs are regulated in the presence of ammonium, we used variable-angle total internal reflection fluorescence microscopy and fluorescence cross-correlation spectroscopy for single-particle fluorescence imaging of EGFP-tagged AMT1;3 on the plasma membrane of Arabidopsis root cells at various ammonium levels. We demonstrated that AMT1;3-EGFP dynamically appeared and disappeared on the plasma membrane as moving fluorescent spots in low oligomeric states under N-deprived and N-sufficient conditions. Under external high-ammonium stress, however, AMT1;3-EGFPs were found to amass into clusters, which were then internalized into the cytoplasm. A similar phenomenon also occurred in the glutamine synthetase mutant gln1;2 background. Single-particle analysis of AMT1;3-EGFPs in the clathrin heavy chain 2 mutant (chc2 mutant) and Flotllin1 artificial microRNA (Flot1 amiRNA) backgrounds, together with chemical inhibitor treatments, demonstrated that the endocytosis of AMT1;3 clusters induced by high-ammonium stress could occur mainly through clathrin-mediated endocytic pathways, but the contribution of microdomain-associated endocytic pathway cannot be excluded in the internalization. Our results revealed that the clustering and endocytosis of AMT1;3 provides an effective mechanism by which plant cells can avoid accumulation of toxic levels of ammonium by eliminating active AMT1;3 from the plasma membrane. |
| 关键词 | VA-TIRFM FCS |
| 学科领域 | Multidisciplinary Sciences |
| DOI | 10.1073/pnas.1301160110 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | PLASMA-MEMBRANE ; DYNAMICS ; ENDOCYTOSIS ; ORGANIZATION ; MICROSCOPY ; MECHANISM ; STEROLS ; PROTEIN ; GROWTH |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000322771100079 |
| 出版者 | NATL ACAD SCIENCES |
| 文献子类 | Article |
| 出版地 | WASHINGTON |
| 资助机构 | 973 Program(National Basic Research Program of China) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of Sciences) ; National Nature Science Foundation of China Project Grant |
| 作者邮箱 | ZHAO, YUAN/HCI-5831-2022 ; De Michele, Roberto/Z-4760-2019 |
| 作品OA属性 | Bronze, Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/28150 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 3.Peking Univ, Hlth Sci Ctr, Beijing 100191, Peoples R China 4.Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA 5.Natl Res Council Italy, Inst Plant Genet, I-90123 Palermo, Italy 6.Leibniz Inst Plant Genet & Crop Plant Res, D-06466 Gatersleben, Germany |
| 推荐引用方式 GB/T 7714 | Wang, Qinli,Zhao, Yuanyuan,Luo, Wangxi,et al. Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(32):13204-13209. |
| APA | Wang, Qinli.,Zhao, Yuanyuan.,Luo, Wangxi.,Li, Ruili.,He, Qihua.,...&Lin, Jinxing.(2013).Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,110(32),13204-13209. |
| MLA | Wang, Qinli,et al."Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.32(2013):13204-13209. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 23882074.pdf（1013KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论