Nuclear DNA content variation of three Miscanthus species in China

doi:10.1007/s13258-013-0063-y

	Nuclear DNA content variation of three Miscanthus species in China
	Li, Xi 1; Hu, Die 1,2; Luo, Manman 1,2; Zhu, Ming 1,2; Li, Xinwei 1; Luo, Fan 3; Li, Jianqiang 1; Yan, Juan 1
	2013
发表期刊	GENES & GENOMICS
ISSN	1976-9571
卷号	35 期号:1 页码:13-20
摘要	In order to estimate the variation in nuclear genome size in Miscanthus, flow cytometry of nuclei stained by propidium iodide was carried out using 36 populations of three Miscanthus species: M. lutarioriparius, M. sacchariflorus and M. sinensis, which were sampled from cold northern to warm and humid southern and central China, as well as near the sea level in eastern China to mountains in western China. The DNA content of diploid was 4.37 +/- A 0.02 pg/2C in M. lutarioriparius, 4.37 +/- A 0.01 pg/2C in M. sacchariflorus, and 5.37 +/- A 0.03 pg/2C in M. sinensis, respectively. There was no intraspecific variation in the three Miscanthus species at the diploid level, suggesting that the genome size was stable within species and the diverse environments did not induce variation in genome size at the diploid level. However, tetraploid populations were found in M. lutarioriparius and M. sacchariflorus, and their genome sizes were 8.56 and 8.54 pg, respectively, which are lower than expected values (8.74 pg), indicating the genome downsizing after polyploidization in the genus. Our results showed that the plant height of M. lutarioriparius was the highest one among the three species and the species was more closely related to M. sacchariflorus than M. sinensis. The intra-species genomic variation and inter-species differentiation in Miscanthus species provide important genetic and genomic information for the development of Miscanthus, especially for the endemic species, M. lutarioriparius, (together with Miscanthus x giganteus) which are now emerging as a key bio-energy crop because of their high yields and strong adaptability.
关键词	Miscanthus Energy crops Polyploidization Genome size Flow cytometry
学科领域	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1007/s13258-013-0063-y
收录类别	SCI
语种	英语
WOS关键词	GENOME SIZE ; INTRASPECIFIC VARIATION ; C-4 PHOTOSYNTHESIS ; ENERGY-CONVERSION ; COLD TOLERANCE ; EVOLUTION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000315345300002
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	2092-9293
资助机构	Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
作者邮箱	luo, man/HGC-7868-2022 ; Luo, Man/GWQ-4789-2022
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28151
专题	系统与进化植物学国家重点实验室
作者单位	1.Fudan Univ, Minist Educ, Key Lab Biodivers Sci & Ecol Engn, Sch Life Sci, Shanghai 200433, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Chinese Acad Sci, State Key Lab Systemat & Evolutionary Bot, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Xi,Hu, Die,Luo, Manman,et al. Nuclear DNA content variation of three Miscanthus species in China[J]. GENES & GENOMICS,2013,35(1):13-20.
APA	Li, Xi.,Hu, Die.,Luo, Manman.,Zhu, Ming.,Li, Xinwei.,...&Yan, Juan.(2013).Nuclear DNA content variation of three Miscanthus species in China.GENES & GENOMICS,35(1),13-20.
MLA	Li, Xi,et al."Nuclear DNA content variation of three Miscanthus species in China".GENES & GENOMICS 35.1(2013):13-20.

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