Proteomic Analysis Points to a Role for RAD23 in Apical Dominance in Pinus sylvestris var. Mongolica

doi:10.1007/s11105-013-0593-0

IB-CAS > 中科院光生物学重点实验室

	Proteomic Analysis Points to a Role for RAD23 in Apical Dominance in Pinus sylvestris var. Mongolica
	Ning, De-Li ; Lu, Tian-Chong ; Liu, Gui-Feng ; Yang, Chuan-Ping ; Wang, Bai-Chen
	2013
发表期刊	PLANT MOLECULAR BIOLOGY REPORTER
ISSN	0735-9640
卷号	31 期号:6 页码:1283-1292
摘要	In higher plants, apical dominance is the phenomenon whereby the main central stem is dominant over other side stems. In the present study, the natural loss of apical dominance in a Pinus sylvestris var. mongolica mutant was characterized. The mutant lacked an obvious trunk, but exhibited a large number of side stems, short internodes and leaves, and dwarfism. Cell biological analyses indicated that the meristem was changed dramatically in the mutant, with irregular cell arrangements, and no obvious layers of meristem and mature tissue. The loss of apical dominance in the mutant plant was accompanied by a significant decrease in auxin (A) and cytokinin (C) content compared to wild type, and increased C/A values. Proteomic analysis of the apical buds using 2DE-ESI-MS/MS at different stages of development (including dormancy, bud break, 10 days after bud break, and 15 days after bud break) revealed 49 proteins that were significantly changed in abundance compared to wild type. These proteins were involved in stress response, energy production and conversion, post-translation modification and amino acid transport and metabolism, as well as many other complex biological processes. Among the identified proteins, Rad23, an abscisic acid- and stress-induced protein, and cytoplasmic aconitate hydratase likely participate in ABA responses, Fe2+ ion homeostasis and the ubiquitin/26S proteasome pathway to regulate apical dominance of buds. Rad23, in particular, appears likely to play an important role in the complicated mechanism of apical dominance of Pinus sylvestris var. mongolica. This work provides important insights into this process as well as forming a basis for the further study of plant apical dominance.
关键词	Apical dominance Rad23 Pinus sylvestris var mongolica mutant 2-DE
学科领域	Biochemical Research Methods ; Plant Sciences
DOI	10.1007/s11105-013-0593-0
收录类别	SCI
语种	英语
WOS关键词	TRANSGENIC TOBACCO PLANTS ; IRON REGULATORY PROTEINS ; ELEMENT-BINDING-PROTEIN ; 26S PROTEASOME ; ARABIDOPSIS GROWTH ; AUXIN RECEPTOR ; DEGRADATION ; ACONITASE ; ACTS ; CYTOKININS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000326804400010
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1572-9818
资助机构	National Natural Science Fund(National Natural Science Foundation of China (NSFC))
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28156
专题	中科院光生物学重点实验室
作者单位	1.[Ning, De-Li 2.Lu, Tian-Chong 3.Liu, Gui-Feng 4.Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China 5.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Ning, De-Li,Lu, Tian-Chong,Liu, Gui-Feng,et al. Proteomic Analysis Points to a Role for RAD23 in Apical Dominance in Pinus sylvestris var. Mongolica[J]. PLANT MOLECULAR BIOLOGY REPORTER,2013,31(6):1283-1292.
APA	Ning, De-Li,Lu, Tian-Chong,Liu, Gui-Feng,Yang, Chuan-Ping,&Wang, Bai-Chen.(2013).Proteomic Analysis Points to a Role for RAD23 in Apical Dominance in Pinus sylvestris var. Mongolica.PLANT MOLECULAR BIOLOGY REPORTER,31(6),1283-1292.
MLA	Ning, De-Li,et al."Proteomic Analysis Points to a Role for RAD23 in Apical Dominance in Pinus sylvestris var. Mongolica".PLANT MOLECULAR BIOLOGY REPORTER 31.6(2013):1283-1292.

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