Analysis of the APETALA3- and PISTILLATA-like genes in Hedyosmum orientale (Chloranthaceae) provides insight into the evolution of the floral homeotic B-function in angiosperms

doi:10.1093/aob/mct182

	Analysis of the APETALA3- and PISTILLATA-like genes in Hedyosmum orientale (Chloranthaceae) provides insight into the evolution of the floral homeotic B-function in angiosperms
	Liu, Shujun 1; Sun, Yonghua 1; Du, Xiaoqiu ; Xu, Qijiang 2; Wu, Feng ; Meng, Zheng
	2013
发表期刊	ANNALS OF BOTANY
ISSN	0305-7364
卷号	112 期号:7 页码:1239-1251
摘要	According to the floral ABC model, B-function genes appear to play a key role in the origin and diversification of the perianth during the evolution of angiosperms. The basal angiosperm Hedyosmum orientale (Chloranthaceae) has unisexual inflorescences associated with a seemingly primitive reproductive morphology and a reduced perianth structure in female flowers. The aim of this study was to investigate the nature of the perianth and the evolutionary state of the B-function programme in this species. A series of experiments were conducted to characterize B-gene homologues isolated from H. orientale, including scanning electron microscopy to observe the development of floral organs, phylogenetic analysis to reconstruct gene evolutionary history, reverse transcriptionPCR, quantitative real-time PCR and in situ hybridization to identify gene expression patterns, the yeast two-hybrid assay to explore protein dimerization affinities, and transgenic analyses in Arabidopsis thaliana to determine activities of the encoded proteins. The expression of HoAP3 genes was restricted to stamens, whereas HoPI genes were broadly expressed in all floral organs. HoAP3 was able to partially restore the stamen but not petal identity in Arabidopsis ap3-3 mutants. In contrast, HoPI could rescue aspects of both stamen and petal development in Arabidopsis pi-1 mutants. When the complete C-terminal sequence of HoPI was deleted, however, no or weak transgenic phenotypes were observed and homodimerization capability was completely abolished. The results suggest that Hedyosmum AP3-like genes have an ancestral function in specifying male reproductive organs, and that the activity of the encoded PI-like proteins is highly conserved between Hedyosmum and Arabidopsis. Moreover, there is evidence that the C-terminal region is important for the function of HoPI. Our findings indicate that the development of the proposed perianth in Hedyosmum does not rely on the B homeotic function.
关键词	Floral homeotic B function Hedyosmum orientale Chloranthaceae C-terminal region HoAP3 HoPI homodimerization APETALA3 PISTILLATA
学科领域	Plant Sciences
DOI	10.1093/aob/mct182
收录类别	SCI
语种	英语
WOS关键词	MADS-BOX GENE ; LILY LILIUM-LONGIFLORUM ; ORGAN IDENTITY ; NEO-FUNCTIONALIZATION ; ECTOPIC EXPRESSION ; ANTIRRHINUM-MAJUS ; CONTROLLING PETAL ; ABC MODEL ; PI MOTIF ; ARABIDOPSIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000326289900011
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1095-8290
资助机构	National Nature Science Foundation of China(National Natural Science Foundation of China (NSFC))
作者邮箱	Sun, Yonghua/B-5025-2012
作品OA属性	Green Published, Bronze
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28159
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Northeast Forestry Univ, Dept Bot, Haerbin 150040, Peoples R China
推荐引用方式 GB/T 7714	Liu, Shujun,Sun, Yonghua,Du, Xiaoqiu,et al. Analysis of the APETALA3- and PISTILLATA-like genes in Hedyosmum orientale (Chloranthaceae) provides insight into the evolution of the floral homeotic B-function in angiosperms[J]. ANNALS OF BOTANY,2013,112(7):1239-1251.
APA	Liu, Shujun,Sun, Yonghua,Du, Xiaoqiu,Xu, Qijiang,Wu, Feng,&Meng, Zheng.(2013).Analysis of the APETALA3- and PISTILLATA-like genes in Hedyosmum orientale (Chloranthaceae) provides insight into the evolution of the floral homeotic B-function in angiosperms.ANNALS OF BOTANY,112(7),1239-1251.
MLA	Liu, Shujun,et al."Analysis of the APETALA3- and PISTILLATA-like genes in Hedyosmum orientale (Chloranthaceae) provides insight into the evolution of the floral homeotic B-function in angiosperms".ANNALS OF BOTANY 112.7(2013):1239-1251.

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