Molecular Characterization and Defoliation-Induced Expression of a Sucrose Transporter LcSUT1 Gene in Sheep Grass (Leymus chinensis)

doi:10.1007/s11105-013-0582-3

	Molecular Characterization and Defoliation-Induced Expression of a Sucrose Transporter LcSUT1 Gene in Sheep Grass (Leymus chinensis)
	Su, Man 1; Li, Xiaoxia 1; Li, Xiaofeng ; Cheng, Liqin ; Qi, Dongmei ; Chen, Shuangyan ; Liu, Gongshe
	2013
发表期刊	PLANT MOLECULAR BIOLOGY REPORTER
ISSN	0735-9640
卷号	31 期号:5 页码:1184-1191
摘要	Sucrose is a major photosynthetic product in plants' leaves. Long-distance transport of sucrose requires sucrose transporter (SUT) to perform loading and unloading functions. In this study, a sucrose transporter gene LcSUT1 was cloned from sheep grass (Leymus chinensis (Trin.) Tzvel), a perennial grass of Gramineae: Poaceae. Bioinformatics analysis showed that the gene product LcSUT1 consisting of 12 predicted transmembrane domains and 11 loops belongs to the SUT1 clade. Heterologous expression of LcSUT1 in yeast proved that it was a functional sucrose transporter. Tissue-specific expression analysis showed that LcSUT1 was highly expressed in leaf and leaf sheath. The expression level of LcSUT1 was significantly up-regulated in leaf sheath after defoliation, but was not induced by wound signal. Furthermore, the level of LcSUT1 expression increased in callus, a model sink tissue, when grew on N6 medium without sucrose. Taken together, our results revealed a novel mechanism in which the increased expression of LcSUT1 in leaf sheaths after defoliation was caused by sucrose starvation rather than by wound signal.
关键词	Sucrose transporter Molecular characterization Sheep grass Sucrose starvation Defoliation
学科领域	Biochemical Research Methods ; Plant Sciences
DOI	10.1007/s11105-013-0582-3
收录类别	SCI
语种	英语
WOS关键词	LOLIUM-PERENNE L ; SIEVE ELEMENTS ; LEAF SHEATHS ; REGROWTH ; CARBON ; FRUCTANS ; NITROGEN ; CARRIER ; FAMILY ; OSSUT1
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000323669900014
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1572-9818
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Ningxia Agricultural Comprehensive Development Office ; Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences) ; National High Technology Research and Development Program of China (863)(National High Technology Research and Development Program of China)
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28166
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Su, Man,Li, Xiaoxia,Li, Xiaofeng,et al. Molecular Characterization and Defoliation-Induced Expression of a Sucrose Transporter LcSUT1 Gene in Sheep Grass (Leymus chinensis)[J]. PLANT MOLECULAR BIOLOGY REPORTER,2013,31(5):1184-1191.
APA	Su, Man.,Li, Xiaoxia.,Li, Xiaofeng.,Cheng, Liqin.,Qi, Dongmei.,...&Liu, Gongshe.(2013).Molecular Characterization and Defoliation-Induced Expression of a Sucrose Transporter LcSUT1 Gene in Sheep Grass (Leymus chinensis).PLANT MOLECULAR BIOLOGY REPORTER,31(5),1184-1191.
MLA	Su, Man,et al."Molecular Characterization and Defoliation-Induced Expression of a Sucrose Transporter LcSUT1 Gene in Sheep Grass (Leymus chinensis)".PLANT MOLECULAR BIOLOGY REPORTER 31.5(2013):1184-1191.

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