Activation of R-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolic O-acetylserine (thiol) lyase in Arabidopsis

doi:10.1111/tpj.12021

	Activation of R-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolic O-acetylserine (thiol) lyase in Arabidopsis
	Tahir, Jibran ; Watanabe, Mutsumi ; Jing, Hai-Chun; Hunter, Donald A.; Tohge, Takayuki ; Nunes-Nesi, Adriano ; Brotman, Yariv ; Fernie, Alisdair R.; Hoefgen, Rainer 7; Dijkwel, Paul P.
	2013
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	73 期号:1 页码:118-130
摘要	O-acetylserine (thiol) lyases (OASTLs) are evolutionarily conserved proteins among many prokaryotes and eukaryotes that perform sulfur acquisition and synthesis of cysteine. A mutation in the cytosolic OASTL-A1 protein ONSET OF LEAF DEATH3 (OLD3) was previously shown to reduce the OASTL activity of the old3-1 protein in vitro and cause auto-necrosis in specific Arabidopsis accessions. Here we investigated why a mutation in this protein causes auto-necrosis in some but not other accessions. The auto-necrosis was found to depend on Recognition of Peronospora Parasitica 1 (RPP1)-like disease resistance R gene(s) from an evolutionarily divergent R gene cluster that is present in Ler-0 but not the reference accession Col-0. RPP1-like gene(s) show a negative epistatic interaction with the old3-1 mutation that is not linked to reduced cysteine biosynthesis. Metabolic profiling and transcriptional analysis further indicate that an effector triggered-like immune response and metabolic disorder are associated with auto-necrosis in old3-1 mutants, probably activated by an RPP1-like gene. However, the old3-1 protein in itself results in largely neutral changes in primary plant metabolism, stress defence and immune responses. Finally, we showed that lack of a functional OASTL-A1 results in enhanced disease susceptibility against infection with virulent and non-virulent Pseudomonas syringae pv. tomato DC3000 strains. These results reveal an interaction between the cytosolic OASTL and components of plant immunity.
关键词	RPP1 OASTL-A1 disease resistance auto-necrosis cysteine biosynthesis innate immunity
学科领域	Plant Sciences
DOI	10.1111/tpj.12021
收录类别	SCI
语种	英语
WOS关键词	CYSTEINE SYNTHASE ; O-ACETYLSERINE(THIOL)LYASE GENE ; SERINE ACETYLTRANSFERASE ; FUNCTIONAL-ANALYSIS ; DEFENSE RESPONSES ; HYDROGEN-PEROXIDE ; BROAD-SPECTRUM ; HRP PILUS ; RESISTANCE ; METABOLISM
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000314183300010
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	Higher Education Commission, Pakistan(Higher Education Commission of Pakistan) ; Massey University Research Fund ; Max Planck Society(Max Planck SocietyFoundation CELLEX)
作者邮箱	Nunes-Nesi, Adriano/D-6430-2013 ; Hunter, Donald A/F-8452-2010 ; Dijkwel, P/J-5515-2013 ; Tohge, Takayuki/G-7924-2017
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28167
专题	中科院北方资源植物重点实验室
作者单位	1.[Tahir, Jibran 2.Dijkwel, Paul P.] Massey Univ, Inst Mol BioSci, Palmerston North, New Zealand 3.[Watanabe, Mutsumi 4.Tohge, Takayuki 5.Nunes-Nesi, Adriano 6.Brotman, Yariv 7.Fernie, Alisdair R. 8.Max Planck Inst Mol Pflanzenphysiol, D-14476 Potsdam, Germany 9.Chinese Acad Sci, Inst Bot, Ctr Bioenergy Plants Res & Dev, Beijing 100093, Peoples R China 10.[Hunter, Donald A.] New Zealand Inst Plant & Food Res Ltd, Palmerston North 4442, New Zealand
推荐引用方式 GB/T 7714	Tahir, Jibran,Watanabe, Mutsumi,Jing, Hai-Chun,et al. Activation of R-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolic O-acetylserine (thiol) lyase in Arabidopsis[J]. PLANT JOURNAL,2013,73(1):118-130.
APA	Tahir, Jibran.,Watanabe, Mutsumi.,Jing, Hai-Chun.,Hunter, Donald A..,Tohge, Takayuki.,...&Dijkwel, Paul P..(2013).Activation of R-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolic O-acetylserine (thiol) lyase in Arabidopsis.PLANT JOURNAL,73(1),118-130.
MLA	Tahir, Jibran,et al."Activation of R-mediated innate immunity and disease susceptibility is affected by mutations in a cytosolic O-acetylserine (thiol) lyase in Arabidopsis".PLANT JOURNAL 73.1(2013):118-130.

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