A stable sandwich' system of Surface-Enhanced Resonance Raman Scattering for the analysis of -carotenes in a photosynthetic pigment-protein complex

doi:10.1002/jrs.4333

IB-CAS > 中科院光生物学重点实验室

	A stable sandwich' system of Surface-Enhanced Resonance Raman Scattering for the analysis of -carotenes in a photosynthetic pigment-protein complex
	Qin, Xiaochun ; Zhu, Jiajia 1; Wang, Wenda 2; Ding, Xiang 1; Wang, Kebin ; Fang, Yan 1; Kuang, Tingyun
	2013
发表期刊	JOURNAL OF RAMAN SPECTROSCOPY
ISSN	0377-0486
卷号	44 期号:8 页码:1111-1119
摘要	In plants, Photosystem I (PSI) is composed of a core complex and a membrane-associated antenna complex light-harvesting complex I that captures light and funnels its energy to the core complex. To obtain Raman structural information on -carotenes embedded in the PSI core complex, a sandwich' system of roughened silver slice: target protein complexes: single silver nanoparticles was fabricated for Surface-Enhanced Resonance Raman Scattering (SERRS) measurements. This study provided a method to overcome spectral irreproducibility, which is the main drawback of Surface-Enhanced Raman Scattering/SERRS-based studies. The Raman spectra of -carotenes embedded in the PSI core complex can be obtained at very low sample concentrations (1-5 mu g Chl/ml) and high signal/noise ratios. The -carotenes in the spinach PSI core complex were predominantly all-trans configuration. The membrane protein-mediated adsorption of silver nanoparticles induced the uniform distribution of a large number of single nanoparticles, which contributed to achieving highly reproducible SERRS spectra. This study is the first to apply single silver nanoparticle-based SERRS analysis in membrane proteins. Copyright (c) 2013 John Wiley & Sons, Ltd.
关键词	carotenoid carotene Photosystem I SERS SERRS
学科领域	Spectroscopy
DOI	10.1002/jrs.4333
收录类别	SCI
语种	英语
WOS关键词	PLANT PHOTOSYSTEM-I ; REACTION CENTERS ; SILVER ; SPECTROSCOPY ; BACTERIAL ; NANOPARTICLES ; MEMBRANES ; SPECTRA ; DISCRIMINATION ; FLUORESCENCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000323180700007
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1097-4555
资助机构	National Natural Science Foundation(National Natural Science Foundation of China (NSFC)) ; National Basic Research Program of China (973 Program)(National Basic Research Program of China)
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28178
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Capital Normal Univ, Beijing Key Lab Nanophoton & Nanostruct, Beijing 100048, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Qin, Xiaochun,Zhu, Jiajia,Wang, Wenda,et al. A stable sandwich' system of Surface-Enhanced Resonance Raman Scattering for the analysis of -carotenes in a photosynthetic pigment-protein complex[J]. JOURNAL OF RAMAN SPECTROSCOPY,2013,44(8):1111-1119.
APA	Qin, Xiaochun.,Zhu, Jiajia.,Wang, Wenda.,Ding, Xiang.,Wang, Kebin.,...&Kuang, Tingyun.(2013).A stable sandwich' system of Surface-Enhanced Resonance Raman Scattering for the analysis of -carotenes in a photosynthetic pigment-protein complex.JOURNAL OF RAMAN SPECTROSCOPY,44(8),1111-1119.
MLA	Qin, Xiaochun,et al."A stable sandwich' system of Surface-Enhanced Resonance Raman Scattering for the analysis of -carotenes in a photosynthetic pigment-protein complex".JOURNAL OF RAMAN SPECTROSCOPY 44.8(2013):1111-1119.

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