Effects of Monogalactoglycerolipid Deficiency and Diacylglycerol Acyltransferase Overexpression on Oil Accumulation in Transgenic Tobacco

doi:10.1007/s11105-013-0574-3

IB-CAS > 中科院光生物学重点实验室

	Effects of Monogalactoglycerolipid Deficiency and Diacylglycerol Acyltransferase Overexpression on Oil Accumulation in Transgenic Tobacco
	Wu, Han-Ying ; Liu, Chao ; Li, Min-Chun ; Zhao, Ming-Ming ; Gu, Dan ; Xu, Yi-Nong
	2013
发表期刊	PLANT MOLECULAR BIOLOGY REPORTER
ISSN	0735-9640
卷号	31 期号:5 页码:1077-1088
摘要	Engineering accumulation of triacylglycerol (TAG) in vegetative tissues has been recently proposed as a promising strategy for increasing plant oil production. However, little is known about regulatory mechanisms involved in increasing oil production in plant vegetative tissues. In this study, expression of NtMGD1 encoding a major biosynthetic enzyme for the chloroplast membrane lipid was inhibited by RNAi interference in tobacco. Furthermore, AtDGAT1, a rate-regulating gene involved in TAG biosynthesis, was ectopically overexpressed. Results showed that leaf TAG accumulations were significantly increased both by NtMGD1 RNAi and AtDGAT1 overexpression. However, combination of AtDGAT1 overexpression with NtMGD1 RNAi did not result in additive increase in TAG accumulation in leaves than AtDGAT1 overexpression or NtMGD1 RNAi alone. In addition, reduction of monogalactosyldiacylglycerol (MGDG) biosynthesis by NtMGD1 RNAi was relieved by AtDGAT1 overexpression. Expression of lipid transfer protein (LTP) was upregulated both by AtDGAT1 overexpression and NtMGD1 RNAi and correlated with increased oil accumulation in leaves. Our results indicated that fatty acids deesterified from chloroplast membrane galactolipids could be redirected into TAG. TAG is an energy-dense molecule that might act as a storage pool for carbohydrate. This membrane lipid remodeling may represent an adaptive response that enables plant cells to avoid toxic effects of free fatty acids.
关键词	Diacylglycerol acyltransferase Monogalactosyldiacylglycerol Nicotiana tabacum Oil accumulation Triacylglycerol
学科领域	Biochemical Research Methods ; Plant Sciences
DOI	10.1007/s11105-013-0574-3
收录类别	SCI
语种	英语
WOS关键词	FATTY-ACID ; TRIACYLGLYCEROLS ; BIOSYNTHESIS ; EXPRESSION ; BREAKDOWN ; LEAVES ; LIPIDS ; GENES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000323669900003
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1572-9818
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Major Special Project of China on New Varieties Cultivation for Transgenic Organisms ; National Key Basic Research Program(National Basic Research Program of China)
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28182
专题	中科院光生物学重点实验室
作者单位	1.[Wu, Han-Ying 2.Liu, Chao 3.Li, Min-Chun 4.Zhao, Ming-Ming 5.Gu, Dan 6.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wu, Han-Ying,Liu, Chao,Li, Min-Chun,et al. Effects of Monogalactoglycerolipid Deficiency and Diacylglycerol Acyltransferase Overexpression on Oil Accumulation in Transgenic Tobacco[J]. PLANT MOLECULAR BIOLOGY REPORTER,2013,31(5):1077-1088.
APA	Wu, Han-Ying,Liu, Chao,Li, Min-Chun,Zhao, Ming-Ming,Gu, Dan,&Xu, Yi-Nong.(2013).Effects of Monogalactoglycerolipid Deficiency and Diacylglycerol Acyltransferase Overexpression on Oil Accumulation in Transgenic Tobacco.PLANT MOLECULAR BIOLOGY REPORTER,31(5),1077-1088.
MLA	Wu, Han-Ying,et al."Effects of Monogalactoglycerolipid Deficiency and Diacylglycerol Acyltransferase Overexpression on Oil Accumulation in Transgenic Tobacco".PLANT MOLECULAR BIOLOGY REPORTER 31.5(2013):1077-1088.

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