Antagonistic Peptide Technology for Functional Dissection of CLV3/ESR Genes in Arabidopsis

doi:10.1104/pp.112.211029

	Antagonistic Peptide Technology for Functional Dissection of CLV3/ESR Genes in Arabidopsis
	Song, Xiu-Fen ; Guo, Peng ; Ren, Shi-Chao ; Xu, Ting-Ting ; Liu, Chun-Ming
	2013
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	161 期号:3 页码:1076-1085
摘要	In recent years, peptide hormones have been recognized as important signal molecules in plants. Genetic characterization of such peptides is challenging since they are usually encoded by small genes. As a proof of concept, we used the well-characterized stem cell-restricting CLAVATA3 (CLV3) to develop an antagonistic peptide technology by transformations of wild-type Arabidopsis (Arabidopsis thaliana) with constructs carrying the full-length CLV3 with every residue in the peptide-coding region replaced, one at a time, by alanine. Analyses of transgenic plants allowed us to identify one line exhibiting a dominant-negative clv3-like phenotype, with enlarged shoot apical meristems and increased numbers of floral organs. We then performed second dimensional amino acid substitutions to replace the glycine residue individually with the other 18 possible proteinaceous amino acids. Examination of transgenic plants showed that a glycine-to-threonine substitution gave the strongest antagonistic effect in the wild type, in which over 70% of transgenic lines showed the clv3-like phenotype. Among these substitutions, a negative correlation was observed between the antagonistic effects in the wild type and the complementation efficiencies in clv3. We also demonstrated that such an antagonistic peptide technology is applicable to other CLV3/EMBRYO SURROUNDING REGION (CLE) genes, CLE8 and CLE22, as well as in vitro treatments. We believe this technology provides a powerful tool for functional dissection of widely occurring CLE genes in plants.
学科领域	Plant Sciences
DOI	10.1104/pp.112.211029
收录类别	SCI
语种	英语
WOS关键词	RECEPTOR-LIKE KINASE ; OF-FUNCTION PHENOTYPES ; MERISTEM MAINTENANCE ; ROOT-MERISTEM ; GROWTH-FACTOR ; CELL FATE ; PLANT ; EXPRESSION ; CLAVATA3 ; THALIANA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000316984100003
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	Ministry of Science and Technology of China(Ministry of Science and Technology, China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Chinese Academy of Sciences(Chinese Academy of Sciences)
作者邮箱	刘, 春明/GLU-4320-2022 ; Liu, Chun-Ming/G-1006-2010 ; guo, peng/AAG-4052-2019 ; Guo, Peng/GWC-0572-2022
作品OA属性	hybrid, Green Published
引用统计	被引频次：60[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28183
专题	中科院植物分子生理学重点实验室
作者单位	1.[Song, Xiu-Fen 2.Guo, Peng 3.Ren, Shi-Chao 4.Xu, Ting-Ting 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 6.[Guo, Peng 7.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Song, Xiu-Fen,Guo, Peng,Ren, Shi-Chao,et al. Antagonistic Peptide Technology for Functional Dissection of CLV3/ESR Genes in Arabidopsis[J]. PLANT PHYSIOLOGY,2013,161(3):1076-1085.
APA	Song, Xiu-Fen,Guo, Peng,Ren, Shi-Chao,Xu, Ting-Ting,&Liu, Chun-Ming.(2013).Antagonistic Peptide Technology for Functional Dissection of CLV3/ESR Genes in Arabidopsis.PLANT PHYSIOLOGY,161(3),1076-1085.
MLA	Song, Xiu-Fen,et al."Antagonistic Peptide Technology for Functional Dissection of CLV3/ESR Genes in Arabidopsis".PLANT PHYSIOLOGY 161.3(2013):1076-1085.

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