Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress

doi:10.1111/jipb.12104

IB-CAS > 中科院光生物学重点实验室

	Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress
	Zhao, Lei 1; Chen, Mei ; Cheng, Dongmei 1; Yang, Haomeng ; Sun, Yongle 1; Zhou, Heyi 1; Huang, Fang
	2013
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
卷号	55 期号:11 页码:1054-1068
摘要	The genome of unicellular green alga Chlamydomonas reinhardtii contains four genes encoding B-type methionine sulfoxide reductases, MSRB1.1, MSRB1.2, MSRB2.1, and MSRB2.2, with functions largely unknown. To understand the cell defense system mediated by the methionine sulfoxide reductases in Chlamydomonas, we analyzed expression and physiological roles of the MSRBs under different abiotic stress conditions using immunoblotting and quantitative polymerase chain reaction (PCR) analyses. We showed that the MSRB2.2 protein was accumulated in cells treated with high light (1,300 mu E/m(2) per s), whereas MSRB1.1 was accumulated in the cells under 1mmol/L H2O2 treatment or sulfur depletion. We observed that the cells with the MSRB2.2 knockdown and overexpression displayed increased and decreased sensitivity to high light, respectively, based on in situ chlorophyll a fluorescence measures. We also observed that the cells with the MSRB1.1 knockdown and overexpression displayed decreased and increased tolerance to sulfur-depletion and oxidative stresses, respectively, based on growth and H-2-producing performance. The physiological implications revealed from the experimental data highlight the importance of MSRB2.2 and MSRB1.1 in protecting Chlamydomonas cells against adverse conditions such as high-light, sulfur-depletion, and oxidative stresses.
关键词	Chlamydomonas high light MSRB oxidative stress sulfur deprivation
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1111/jipb.12104
收录类别	SCI
语种	英语
WOS关键词	MACROALGA ULVA-FASCIATA ; PHOTOSYSTEM-II ; PHOTOOXIDATIVE STRESS ; ARABIDOPSIS ; REINHARDTII ; EXPRESSION ; EVOLUTION ; MAMMALS ; MSRA ; PHOTOSYNTHESIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000328148600006
出版者	WILEY-BLACKWELL
文献子类	Article
出版地	HOBOKEN
EISSN	1744-7909
资助机构	Ministry of Science and Technology of China(Ministry of Science and Technology, China) ; Chinese Ministry of Agriculture(Ministry of Agriculture & Rural Affairs) ; Chinese Academy of Sciences(Chinese Academy of Sciences)
作者邮箱	Huang, fangcheng/GRK-0079-2022 ; Cheng, Dongmei/N-8997-2015
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28186
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Lei,Chen, Mei,Cheng, Dongmei,et al. Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2013,55(11):1054-1068.
APA	Zhao, Lei.,Chen, Mei.,Cheng, Dongmei.,Yang, Haomeng.,Sun, Yongle.,...&Huang, Fang.(2013).Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,55(11),1054-1068.
MLA	Zhao, Lei,et al."Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 55.11(2013):1054-1068.

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