CYCLIN H;1 Regulates Drought Stress Responses and Blue Light-Induced Stomatal Opening by Inhibiting Reactive Oxygen Species Accumulation in Arabidopsis

doi:10.1104/pp.113.215798

	CYCLIN H;1 Regulates Drought Stress Responses and Blue Light-Induced Stomatal Opening by Inhibiting Reactive Oxygen Species Accumulation in Arabidopsis
	Zhou, Xiao Feng ; Jin, Yin Hua ; Yoo, Chan Yul ; Lin, Xiao-Li ; Kim, Woe-Yeon ; Yun, Dae-Jin ; Bressan, Ray A.; Hasegawa, Paul M.; Jin, Jing Bo 3
	2013
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	162 期号:2 页码:1030-1041
摘要	Arabidopsis (Arabidopsis thaliana) CYCLIN-DEPENDENT KINASE Ds (CDKDs) phosphorylate the C-terminal domain of the largest subunit of RNA polymerase II. Arabidopsis CYCLIN H; 1 (CYCH;1) interacts with and activates CDKDs; however, the physiological function of CYCH; 1 has not been determined. Here, we report that CYCH; 1, which is localized to the nucleus, positively regulates blue light-induced stomatal opening. Reduced-function cych;1 RNA interference (cych; 1 RNAi) plants exhibited a drought tolerance phenotype. CYCH; 1 is predominantly expressed in guard cells, and its expression was substantially down-regulated by dehydration. Transpiration of intact leaves was reduced in cych; 1 RNAi plants compared with the wild-type control in light but not in darkness. CYCH; 1 down-regulation impaired blue light-induced stomatal opening but did not affect guard cell development or abscisic acid-mediated stomatal closure. Microarray and real-time polymerase chain reaction analyses indicated that CYCH; 1 did not regulate the expression of abscisic acid-responsive genes or light-induced stomatal opening signaling determinants, such as MYB60, MYB61, Hypersensitive to red and blue1, and Protein phosphatase7. CYCH; 1 down-regulation induced the expression of redox homeostasis genes, such as LIPOXYGENASE3 (LOX3), LOX4, ARABIDOPSIS GLUTATHIONE PEROXIDASE 7 (ATGPX7), EARLY LIGHT-INDUCIBLE PROTEIN1 (ELIP1), and ELIP2, and increased hydrogen peroxide production in guard cells. Furthermore, loss-of-function mutations in CDKD; 2 or CDKD; 3 did not affect responsiveness to drought stress, suggesting that CYCH; 1 regulates the drought stress response in a CDKD-independent manner. We propose that CYCH; 1 regulates blue light-mediated stomatal opening by controlling reactive oxygen species homeostasis.
学科领域	Plant Sciences
DOI	10.1104/pp.113.215798
收录类别	SCI
语种	英语
WOS关键词	CDK-ACTIVATING KINASE ; MEMBRANE H+-ATPASE ; TRANSCRIPTION FACTOR TFIIH ; ABSCISIC-ACID ; GUARD-CELL ; ABIOTIC STRESSES ; PROTEINS ; PHOSPHORYLATION ; TOLERANCE ; THALIANA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000319819900039
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
资助机构	Ministry of Science and Technology(Ministry of Science, ICT & Future Planning, Republic of Korea) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Chinese Academy of Sciences (Hundred Talents Program)(Chinese Academy of Sciences)
作者邮箱	YOO, CHAN YUL/D-8399-2017
作品OA属性	hybrid, Green Published
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28188
专题	中科院植物分子生理学重点实验室
作者单位	1.[Zhou, Xiao Feng 2.Jin, Yin Hua 3.Lin, Xiao-Li 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 5.[Yoo, Chan Yul 6.Bressan, Ray A. 7.Hasegawa, Paul M.] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA 8.[Kim, Woe-Yeon 9.Gyeongsang Natl Univ, Environm Biotechnol Natl Core Res Ctr, Div Appl Life Sci, Brain Korea Program 21, Jinju 660701, South Korea
推荐引用方式 GB/T 7714	Zhou, Xiao Feng,Jin, Yin Hua,Yoo, Chan Yul,et al. CYCLIN H;1 Regulates Drought Stress Responses and Blue Light-Induced Stomatal Opening by Inhibiting Reactive Oxygen Species Accumulation in Arabidopsis[J]. PLANT PHYSIOLOGY,2013,162(2):1030-1041.
APA	Zhou, Xiao Feng.,Jin, Yin Hua.,Yoo, Chan Yul.,Lin, Xiao-Li.,Kim, Woe-Yeon.,...&Jin, Jing Bo.(2013).CYCLIN H;1 Regulates Drought Stress Responses and Blue Light-Induced Stomatal Opening by Inhibiting Reactive Oxygen Species Accumulation in Arabidopsis.PLANT PHYSIOLOGY,162(2),1030-1041.
MLA	Zhou, Xiao Feng,et al."CYCLIN H;1 Regulates Drought Stress Responses and Blue Light-Induced Stomatal Opening by Inhibiting Reactive Oxygen Species Accumulation in Arabidopsis".PLANT PHYSIOLOGY 162.2(2013):1030-1041.

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