Tip growth defective1 interacts with cellulose synthase A3 to regulate cellulose biosynthesis in Arabidopsis

doi:10.1007/s11103-022-01283-8

	Tip growth defective1 interacts with cellulose synthase A3 to regulate cellulose biosynthesis in Arabidopsis
	Zhang, Lu 1; Thapa Magar, Madhu Shudan 1,2; Wang, Yanning 1; Cheng, Youfa1,3
	2022
发表期刊	PLANT MOLECULAR BIOLOGY
ISSN	0167-4412
卷号	110 期号:1-2 页码:1-12
摘要	Key message AtTIP1 physically and genetically interacts with AtCESA3. AtCESA3 undergoes S-acylation, possibly mediated by AtTIP1, suggesting a specific role of AtTIP1 in cellulose biosynthesis and plant development. S-acylation is a reversible post-translational lipid modification of proteins catalyzed by protein S-acyl transferases (PATs). S-acylation is important for various biological molecular mechanisms including cellulose biosynthesis. Cellulose is synthesized by the cellulose synthase A (CESA) complexes (CSCs) at the plasma membrane. However, specific PAT involving in cellulose biosynthesis has not been identified and the precise mechanism by which PAT regulates the CESAs is largely unknown. Here, we report isolation of tip1-5, an allele of Tip Growth Defective1 (AtTIP1/AtPAT24) with a premature stop codon. tip1-5 genetically interacts with ixr1-2, a point mutant of AtCESA3 which encodes a catalytic subunit of CSC synthesizing primary wall cellulose. We show that AtTIP1 physically interacts with AtCESA3. AtCESA3 undergoes S-acylation, which is possibly mediated by AtTIP1, suggesting a functional relationship between AtTIP1 and AtCESA3. Moreover, the interfascicular fiber cells in the primary inflorescence stems of tip1-5 ixr1-2 double mutant contain thinner cell walls and significantly less crystalline cellulose compared to the single mutants. These results highlight the positive regulation of AtTIP1 in cellulose biosynthesis, and a specific role of AtPAT in plant development.
关键词	S-acylation Protein S-acyl transferases CESA3 Cellulose biosynthesis AtTIP1 AtPAT24
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1007/s11103-022-01283-8
收录类别	SCI
语种	英语
WOS关键词	PROTEIN ; COMPLEXES ; MUTANT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000802059600001
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5028
资助机构	National Natural Science Foundation of China (NSFC) [31970309, 31171389] ; National Key Research and Development Program of China [2016YFD0100400] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA26030302] ; STS Program of the Chinese Academy of Sciences [KFJ-STS-ZDTP-076] ; CAS-TWAS President's Fellowship program
作者邮箱	yfcheng@ibcas.ac.cn
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28463
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Govt Nepal, Dept Plant Resources, Minist Forests & Environm, Kathmandu 44600, Nepal 4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lu,Thapa Magar, Madhu Shudan,Wang, Yanning,et al. Tip growth defective1 interacts with cellulose synthase A3 to regulate cellulose biosynthesis in Arabidopsis[J]. PLANT MOLECULAR BIOLOGY,2022,110(1-2):1-12.
APA	Zhang, Lu,Thapa Magar, Madhu Shudan,Wang, Yanning,&Cheng, Youfa.(2022).Tip growth defective1 interacts with cellulose synthase A3 to regulate cellulose biosynthesis in Arabidopsis.PLANT MOLECULAR BIOLOGY,110(1-2),1-12.
MLA	Zhang, Lu,et al."Tip growth defective1 interacts with cellulose synthase A3 to regulate cellulose biosynthesis in Arabidopsis".PLANT MOLECULAR BIOLOGY 110.1-2(2022):1-12.

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