Knowledge Management System Of Institute Of Botany,CAS
| MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple | |
| Dong, Haiqiang; Zheng, Qingbo2; Zhou, Yufei; Zhou, Yuwen; Bao, Zeyang; Lan, Qingqing; Li, Xu1 | |
| 2022 | |
| Source Publication | PLANT PHYSIOLOGY AND BIOCHEMISTRY
 |
| ISSN | 0981-9428 |
| Volume | 186Pages:11-18 |
| Abstract | Apple (Malus domestica Borkh.) is not only an important fruit crop distributed worldwide, but also a common model plant. However, the lack of efficient genetic transformation procedures for apples limits the in-depth studies of their gene functions. Although leaf-regenerated adventitious shoots (LRAS) are a prerequisite for successful genetic transformation of apple, little is known about the underlying molecular mechanism of LRAS. Here, we identified the WUSCHEL-related homeobox (WOX) transcription factor in apple, MdWOX4-2, which was a transcriptional activator. Gene expression as well as morphological and histological observations revealed that MdWOX4-2 is involved in the development of LRAS. Overexpression of MdWOX4-2 conferred higher regenerative capacity in transgenic tobacco (Nicotiana tabacum) as compared to the wild type (WT). The com-bined results of the yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), dual luciferase assays, and transient transactivation assay, revealed that MdWOX4-2 directly bound to and activated the MdLBD41 promoter. Moreover, transgenic experiments further demonstrated that MdLBD41 could significantly enhance the formation of adventitious shoot in transgenic tobacco. Collectively, our findings demonstrate that MdWOX4-2 is important for regulating the LRAS development by activating MdLBD41. |
| Keyword | Apple Adventitious shoot Regeneration MdWOX4-2 MdLBD41 |
| Subject Area | Plant Sciences |
| DOI | 10.1016/j.plaphy.2022.06.026 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | LATERAL ROOT-FORMATION ; WUSCHEL-RELATED HOMEOBOX4 ; CELL-PROLIFERATION ; GENE ; REGENERATION ; LEAF ; WOX4 ; DOWNSTREAM ; ACTIVATION ; REPRESSION |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000830070200002 |
| Publisher | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| Subtype | Article |
| Publication Place | ISSY-LES-MOULINEAUX |
| EISSN | 1873-2690 |
| Funding Organization | Zhejiang Provincial Top Discipline of Biological Engineering [(Level A) (ZS2021003), KF2022008] ; China Postdoctoral Science Foundation [2022M713004] |
| Corresponding Author Email | ybulixu@163.com |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/28466 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Zhejiang Wanli Univ, Coll Biol & Environm Sci, Ningbo 315100, Zhejiang, Peoples R China 2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China 3.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 10093, Peoples R China |
| Recommended Citation GB/T 7714 | Dong, Haiqiang,Zheng, Qingbo,Zhou, Yufei,et al. MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2022,186:11-18. |
| APA | Dong, Haiqiang.,Zheng, Qingbo.,Zhou, Yufei.,Zhou, Yuwen.,Bao, Zeyang.,...&Li, Xu.(2022).MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple.PLANT PHYSIOLOGY AND BIOCHEMISTRY,186,11-18. |
| MLA | Dong, Haiqiang,et al."MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple".PLANT PHYSIOLOGY AND BIOCHEMISTRY 186(2022):11-18. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| MdWOX4_2_modulated_M（3548KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment