Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants

doi:10.1080/10409238.2021.1979458

	Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants
	Wang, Jing 1,2; Guo, Yanhong 1; Yin, Xue 1; Wang, Xiaoning 3; Qi, Xiaoquan4 ; Xue, Zheyong 1
	2022
发表期刊	CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN	1040-9238
卷号	57 期号:2 页码:113-132
摘要	Triterpenoids are one of the largest groups of secondary metabolites and exhibit diverse structures, which are derived from C30 skeletons that are biosynthesized via the isoprenoid pathway by cyclization of 2,3-oxidosqualene. Triterpenoids have a wide range of biological activities, and are used in functional foods, drugs, and as industrial materials. Due to the low content levels in their native plants and limited feasibility and efficiency of chemical synthesis, heterologous biosynthesis of triterpenoids is the most promising strategy. Herein, we classified 121 triterpene alcohols/ketones according to their conformation and ring numbers, among which 51 skeletons have been experimentally characterized as the products of oxidosqualene cyclases (OSCs). Interestingly, 24 skeletons that have not been reported from nature source were generated by OSCs in heterologous expression. Comprehensive evolutionary analysis of the identified 152 OSCs from 75 species in 25 plant orders show that several pentacyclic triterpene synthases repeatedly originated in multiple plant lineages. Comparative analysis of OSC catalytic reaction revealed that stabilization of intermediate cations, steric hindrance, and conformation of active center amino acid residues are primary factors affecting triterpene formation. Optimization of OSC could be achieved by changing of side-chain orientations of key residues. Recently, methods, such as rationally design of pathways, regulation of metabolic flow, compartmentalization engineering, etc., were introduced in improving chassis for the biosynthesis of triterpenoids. We expect that extensive study of natural variation of large number of OSCs and catalytical mechanism will provide basis for production of high level of triterpenoids by application of synthetic biology strategies.
关键词	Triterpene oxidosqualene cyclase functional divergence catalytic mechanism biosynthesis
学科领域	Biochemistry & Molecular Biology
DOI	10.1080/10409238.2021.1979458
收录类别	SCI
语种	英语
WOS关键词	BETA-AMYRIN SYNTHASE ; OXIDOSQUALENE-LANOSTEROL CYCLASE ; ENCODING CYCLOARTENOL SYNTHASE ; CELL-SUSPENSION CULTURES ; SACCHAROMYCES-CEREVISIAE ; MOLECULAR-CLONING ; FUNCTIONAL EXPRESSION ; LUPEOL SYNTHASE ; STERIC BULK ; CATALYTIC ACTION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000702951600001
出版者	TAYLOR & FRANCIS LTD
文献子类	Review
出版地	ABINGDON
EISSN	1549-7798
资助机构	National Natural Science Foundation of China including Major International (Regional) Joint Research Project and General Project [NSFC] [31920103003, 31770332, 31970314] ; Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team)
作者邮箱	wangxn@sdu.edu.cn ; xqi@ibcas.ac.cn ; xuezhy@126.com
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28477
专题	中科院植物分子生理学重点实验室
作者单位	1.Northeast Forestry Univ, Minist Educ, Key Lab Saline Alkali Vegetat Ecol Restorat, Harbin, Peoples R China 2.Northeast Forestry Univ, Heilongjiang Key Lab Plant Bioact Subst Biosynthe, Harbin, Peoples R China 3.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing, Peoples R China 4.Shandong Univ, Sch Pharmaceut Sci, Minist Educ, Dept Nat Prod Chem,Key Lab Chem Biol, Jinan, Peoples R China 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jing,Guo, Yanhong,Yin, Xue,et al. Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants[J]. CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY,2022,57(2):113-132.
APA	Wang, Jing,Guo, Yanhong,Yin, Xue,Wang, Xiaoning,Qi, Xiaoquan,&Xue, Zheyong.(2022).Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants.CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY,57(2),113-132.
MLA	Wang, Jing,et al."Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants".CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY 57.2(2022):113-132.

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