The methyl-CpG-binding domain family member PEM1 is essential for Ubisch body formation and pollen exine development in rice

doi:10.1111/tpj.15887

	The methyl-CpG-binding domain family member PEM1 is essential for Ubisch body formation and pollen exine development in rice
	Song, Yunyun 1; Tang, Yongyan ; Liu, Lingtong ; Xu, Yunyuan 1,2; Wang, Tai1,2
	2022
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	111 期号:5 页码:1283-1295
摘要	Pollen exine is composed of finely-organized nexine, bacula and tectum, and is crucial for pollen viability and function. Pollen exine development involves a complicated molecular network that coordinates the interaction between pollen and tapetal cells, as well as the biosynthesis, transport and assembly of sporopollenin precursors; however, our understanding of this network is very limited. Here, we report the roles of PEM1, a member of methyl-CpG-binding domain family, in rice pollen development. PEM1 expressed constitutively and, in anthers, its expression was detectable in tapetal cells and pollen. This predicted PEM1 protein of 240 kDa had multiple epigenetic-related domains. pem1 mutants exhibited abnormal Ubisch bodies, delayed exine occurrence and, finally, defective exine, including invisible bacula, amorphous and thickened nexine and tectum layer structures, and also had the phenotype of increased anther cuticle. The mutation in PEM1 did not affect the timely degradation of tapetum. Lipidomics revealed much higher wax and cutin contents in mutant anthers than in wild-type. Accordingly, this mutation up-regulated the expression of a set of genes implicated in transcriptional repression, signaling and diverse metabolic pathways. These results indicate that PEM1 mediates Ubisch body formation and pollen exine development mainly by negatively modulating the expression of genes. Thus, the PEM1-mediated molecular network represents a route for insights into mechanisms underlying pollen development. PEM1 may be a master regulator of pollen exine development.
关键词	MBD protein Ubisch body pollen exine pollen Oryza sativa
学科领域	Plant Sciences
DOI	10.1111/tpj.15887
收录类别	SCI
语种	英语
WOS关键词	TRANSCRIPTION FACTOR ; TAPETUM DEVELOPMENT ; DNA METHYLATION ; GENETIC PATHWAY ; FLOWERING TIME ; MALE-STERILITY ; WALL FORMATION ; MALE-FERTILITY ; EXPRESSION ; PROTEIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000823286900001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	Chinese Academy of Sciences [XDA24010103] ; National Natural Science Foundation of China [32000247]
作者邮箱	twang@ibcas.ac.cn
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28489
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Song, Yunyun,Tang, Yongyan,Liu, Lingtong,et al. The methyl-CpG-binding domain family member PEM1 is essential for Ubisch body formation and pollen exine development in rice[J]. PLANT JOURNAL,2022,111(5):1283-1295.
APA	Song, Yunyun,Tang, Yongyan,Liu, Lingtong,Xu, Yunyuan,&Wang, Tai.(2022).The methyl-CpG-binding domain family member PEM1 is essential for Ubisch body formation and pollen exine development in rice.PLANT JOURNAL,111(5),1283-1295.
MLA	Song, Yunyun,et al."The methyl-CpG-binding domain family member PEM1 is essential for Ubisch body formation and pollen exine development in rice".PLANT JOURNAL 111.5(2022):1283-1295.

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