Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches

doi:10.1111/tpj.15660

	Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches
	Xu, Hui ; Li, Zhen 2,3; Jiang, Peng-Fei ; Zhao, Li 4; Qu, Chang ; Van de Peer, Yves 2,3,5; Liu, Yan-Jing ; Zeng, Qing-Yin
	2022
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	110 期号:1 页码:129-146
摘要	Enzymes are essential components of all biological systems. The key characteristics of proteins functioning as enzymes are their substrate specificities and catalytic efficiencies. In plants, most genes encoding enzymes are members of large gene families. Within such families, the contributions of active site motifs to the functional divergence of duplicate genes have not been well elucidated. In this study, we identified 41 glutaredoxin (GRX) genes in the Populus trichocarpa genome. GRXs are ubiquitous enzymes in plants that play important roles in developmental and stress tolerance processes. In poplar, GRX genes were divided into four classes based on clear differences in gene structure and expression pattern, subcellular localization, enzymatic activity, and substrate specificity of the encoded proteins. Using site-directed mutagenesis, this study revealed that the divergence of the active site motif among different classes of GRX proteins resulted in substrate switches and thus provided new insights into the molecular evolution of these important plant enzymes.
关键词	active site motif gene family functional divergence glutaredoxin enzymatic specificity Populus trichocarpa
学科领域	Plant Sciences
DOI	10.1111/tpj.15660
收录类别	SCI
语种	英语
WOS关键词	ARABIDOPSIS CHLOROPLASTIC GLUTAREDOXIN ; GENE FAMILY ; SUBCELLULAR RELOCALIZATION ; FUNCTIONAL DIVERGENCE ; CATALYTIC-PROPERTIES ; YEAST GLUTAREDOXINS ; ESCHERICHIA-COLI ; SUPERGENE FAMILY ; DUPLICATED GENES ; REDOX REGULATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000759059500001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Science Foundation of China [31822011, 31425006] ; Chinese Academy of Forestry [CAFYBB2018ZX001] ; Special Research Fund of Ghent University [BOFPDO2018001701]
作者邮箱	yan.jing.liu@163.com ; qingyin.zeng@caf.ac.cn
作品OA属性	Green Published
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28491
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 3.Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium 4.VIB Ctr Plant Syst Biol, B-9052 Ghent, Belgium 5.Umea Univ, Dept Ecol & Environm Sci, SE-90187 Umea, Sweden 6.Univ Pretoria, Dept Biochem Genet & Microbiol, ZA-0028 Pretoria, South Africa
推荐引用方式 GB/T 7714	Xu, Hui,Li, Zhen,Jiang, Peng-Fei,et al. Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches[J]. PLANT JOURNAL,2022,110(1):129-146.
APA	Xu, Hui.,Li, Zhen.,Jiang, Peng-Fei.,Zhao, Li.,Qu, Chang.,...&Zeng, Qing-Yin.(2022).Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches.PLANT JOURNAL,110(1),129-146.
MLA	Xu, Hui,et al."Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches".PLANT JOURNAL 110.1(2022):129-146.

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