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| Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1 | |
| Wang, Jia; Li, Xiaoyi1; Chang, Jing-Wen; Ye, Tong; Mao, Ying; Wang, Xiao; Liu, Lin | |
| 2022 | |
| Source Publication | FEBS OPEN BIO
 |
| ISSN | 2211-5463 |
| Volume | 12Issue:9Pages:1677-1687 |
| Abstract | Arabidopsis thaliana heme oxygenase-1 (AtHO-1), a metabolic enzyme in the heme degradation pathway, serves as a prototype for study of the bilin-related functions in plants. Past biological analyses revealed that AtHO-1 requires ferredoxin-NADP(+) reductase (FNR) and ferredoxin for its enzymatic activity. Here, we characterized the binding and degradation of heme by AtHO-1, and found that ferredoxin is a dispensable component of the reducing system that provides electrons for heme oxidation. Furthermore, we reported the crystal structure of heme-bound AtHO-1, which demonstrates both conserved and previously undescribed features of plant heme oxygenases. Finally, the electron transfer pathway from FNR to AtHO-1 is suggested based on the known structural information. |
| Keyword | Arabidopsis thaliana biliverdin crystallography heme heme oxygenase |
| Subject Area | Biochemistry & Molecular Biology |
| DOI | 10.1002/2211-5463.13453 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | PHYTOCHROME-CHROMOPHORE BIOSYNTHESIS ; SYNECHOCYSTIS SP PCC-6803 ; BOUND CRYSTAL-STRUCTURES ; RAT HEME ; CORYNEBACTERIUM-DIPHTHERIAE ; CATALYTIC MECHANISM ; ELECTRON-TRANSFER ; PLANT HEME ; COMPLEX ; MUTANT |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000813393200001 |
| Publisher | WILEY |
| Subtype | Article |
| Publication Place | HOBOKEN |
| Funding Organization | National Natural Science Foundation of China [31800251, 31870729] ; National Key R&D Program of China [2017YFA0503703] ; Anhui Provincial Natural Science Foundation [1908085QC129] ; Undergraduate Innovation and Entrepreneurship Training Program [X202110357056, 202110357051] |
| Corresponding Author Email | liulin@ahu.edu.cn |
| OA | Green Published |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/28517 |
| Collection | 中科院光生物学重点实验室 |
| Affiliation | 1.Anhui Univ, Sch Life Sci, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Jia,Li, Xiaoyi,Chang, Jing-Wen,et al. Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1[J]. FEBS OPEN BIO,2022,12(9):1677-1687. |
| APA | Wang, Jia.,Li, Xiaoyi.,Chang, Jing-Wen.,Ye, Tong.,Mao, Ying.,...&Liu, Lin.(2022).Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1.FEBS OPEN BIO,12(9),1677-1687. |
| MLA | Wang, Jia,et al."Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1".FEBS OPEN BIO 12.9(2022):1677-1687. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Wang-2022-Enzymologi（5336KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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