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Two chloroplast-localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis | |
Yuan, Jiarui1; Ma, Tingting; Ji, Shuiling2; Hedtke, Boris2; Grimm, Bernhard2; Lin, Rongcheng1 | |
2022 | |
发表期刊 | NEW PHYTOLOGIST |
ISSN | 0028-646X |
卷号 | 235期号:5页码:1868-1883 |
摘要 | Tetrapyrroles have essential functions as pigments and cofactors during plant growth and development, and the tetrapyrrole biosynthesis pathway is tightly controlled. Multiple organellar RNA editing factors (MORFs) are required for editing of a wide variety of RNA sites in chloroplasts and mitochondria, but their biochemical properties remain elusive. Here, we uncovered the roles of chloroplast-localized MORF2 and MORF9 in modulating tetrapyrrole biosynthesis and embryogenesis in Arabidopsis thaliana. The lack or reduced transcripts of MORF2 or MORF9 significantly affected biosynthesis of the tetrapyrrole precursor 5-aminolevulinic acid and accumulation of Chl and other tetrapyrrole intermediates. MORF2 directly interacts with multiple tetrapyrrole biosynthesis enzymes and regulators, including NADPH:PROTOCHLOROPHYLLIDE OXIDOREDUCTASE B (PORB) and GENOMES UNCOUPLED4 (GUN4). Strikingly, MORF2 and MORF9 display holdase chaperone activity, alleviate the aggregation of PORB in vitro, and are essential for POR accumulation in vivo. Moreover, both MORF2 and MORF9 significantly stimulate magnesium chelatase activity. Our findings reveal a previously unknown biochemical property of MORF proteins as chaperones and point to a new layer of post-translational control of the tightly regulated tetrapyrrole biosynthesis in plants. |
关键词 | chaperone GUN4 MORF POR tetrapyrrole biosynthesis |
学科领域 | Plant Sciences |
DOI | 10.1111/nph.18273 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | NADPH-PROTOCHLOROPHYLLIDE OXIDOREDUCTASE ; CHLOROPHYLL BIOSYNTHESIS ; POSTTRANSLATIONAL CONTROL ; ARABIDOPSIS ; CHELATASE ; PORPHOBILINOGEN ; IDENTIFICATION ; STABILIZATION ; EMBRYOGENESIS ; EXPRESSION |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000819564400001 |
出版者 | WILEY |
文献子类 | Article |
出版地 | HOBOKEN |
EISSN | 1469-8137 |
资助机构 | National Key R&D Program of China [2019YFA0904600, 2020YFA0907600] ; National Natural Science Foundation of China [31870213] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27030205] ; Chinese Scholarship Council ; Deutsche Forschungsgemeinschaft [SFB TRR175] |
作者邮箱 | rclin@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/28529 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Humboldt Univ, Inst Biol Plant Physiol, D-10099 Berlin, Germany |
推荐引用方式 GB/T 7714 | Yuan, Jiarui,Ma, Tingting,Ji, Shuiling,et al. Two chloroplast-localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis[J]. NEW PHYTOLOGIST,2022,235(5):1868-1883. |
APA | Yuan, Jiarui,Ma, Tingting,Ji, Shuiling,Hedtke, Boris,Grimm, Bernhard,&Lin, Rongcheng.(2022).Two chloroplast-localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis.NEW PHYTOLOGIST,235(5),1868-1883. |
MLA | Yuan, Jiarui,et al."Two chloroplast-localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis".NEW PHYTOLOGIST 235.5(2022):1868-1883. |
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1705385823_62e812d52(3519KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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