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Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus | |
Zhu, Qingjun1; Yang, Yanyan; Xiao, Yanan1; Han, Wenhui1; Li, Xingyue1; Wang, Wenda; Kuang, Tingyun; Shen, Jian-Ren; Han, Guangye | |
2022 | |
发表期刊 | PHOTOSYNTHESIS RESEARCH |
ISSN | 0166-8595 |
卷号 | 152期号:2页码:193-206 |
摘要 | Photosystem II (PSII) has a number of hydrogen-bonding networks connecting the manganese cluster with the lumenal bulk solution. The structure of PSII from Thermosynechococcus vulcanus (T. vulcanus) showed that D1-R323, D1-N322, D1-D319 and D1-H304 are involved in one of these hydrogen-bonding networks located in the interfaces between the D1, CP43 and PsbV subunits. In order to investigate the functions of these residues in PSII, we generated seven site-directed mutants D1-R323A, D1-R323E, D1-N322R, D1-D319L, D1-D319R, D1-D319Y and D1-H304D of T. vulcanus and examined the effects of these mutations on the growth and functions of the oxygen-evolving complex. The photoautotrophic growth rates of these mutants were similar to that of the wild type, whereas the oxygen-evolving activities of the mutant cells were decreased differently to 63-91% of that of the wild type at pH 6.5. The mutant cells showed a higher relative activity at higher pH region than the wild type cells, suggesting that higher pH facilitated proton egress in the mutants. In addition, oxygen evolution of thylakoid membranes isolated from these mutants showed an apparent decrease compared to that of the cells. This is due to the loss of PsbU during purification of the thylakoid membranes. Moreover, PsbV was also lost in the PSII core complexes purified from the mutants. Taken together, D1-R323, D1-N322, D1-D319 and D1-H304 are vital for the optimal function of oxygen evolution and functional binding of extrinsic proteins to PSII core, and may be involved in the proton egress pathway mediated by Y-Z. |
关键词 | Photosystem II Site-directed mutagenesis Water oxidation Hydrogen-bond networks Functional binding |
学科领域 | Plant Sciences |
DOI | 10.1007/s11120-022-00920-z |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | PHOTOSYNTHETIC WATER OXIDATION ; ELECTRON-TRANSFER ; MANGANESE CLUSTER ; 12-KDA PROTEIN ; PROTON RELEASE ; O2 EVOLUTION ; D1 PROTEIN ; OXYGEN ; MECHANISM ; BINDING |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000790102300001 |
出版者 | SPRINGER |
Special Issue | SI |
文献子类 | Article |
出版地 | DORDRECHT |
EISSN | 1573-5079 |
资助机构 | National Natural Science Foundation of China [31470339] ; National Key R&D Program of China [2017YFA0503700, 2020YFA0907600] ; CAS Project for Young Scientists in Basic Research [YSBR-004] ; CAS Interdisciplinary Innovation Team [JCTD-2020-06] ; Strategic Priority Research Program of CAS [XDA26050402] |
作者邮箱 | shen@cc.okayama-u.ac.jp ; hanguangye@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/28534 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, 1 Beichen West Rd, Beijing 100101, Peoples R China 4.Okayama Univ, Inst Interdisciplinary Sci, Grad Sch Nat Sci & Technol, Tsushima Naka 3-1-1, Okayama 7008530, Japan |
推荐引用方式 GB/T 7714 | Zhu, Qingjun,Yang, Yanyan,Xiao, Yanan,et al. Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus[J]. PHOTOSYNTHESIS RESEARCH,2022,152(2):193-206. |
APA | Zhu, Qingjun.,Yang, Yanyan.,Xiao, Yanan.,Han, Wenhui.,Li, Xingyue.,...&Han, Guangye.(2022).Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus.PHOTOSYNTHESIS RESEARCH,152(2),193-206. |
MLA | Zhu, Qingjun,et al."Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus".PHOTOSYNTHESIS RESEARCH 152.2(2022):193-206. |
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