NADP+ supply adjusts the synthesis of photosystem I in Arabidopsis chloroplasts

doi:10.1093/plphys/kiac161

IB-CAS > 中科院光生物学重点实验室

	NADP+ supply adjusts the synthesis of photosystem I in Arabidopsis chloroplasts
	Ji, Daili ; Li, Qiuxin 1; Guo, Yinjie 1; An, Wenjing 1; Manavski, Nikolay 2; Meurer, Jorg 2; Chi, Wei 1,3
	2022
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	189 期号:4 页码:2128-2143
摘要	In oxygenic photosynthesis, NADP(+) acts as the final acceptor of the photosynthetic electron transport chain and receives electrons via the thylakoid membrane complex photosystem I (PSI) to synthesize NAPDH by the enzyme ferredoxin:NADP(+) oxidoreductase. The NADP(+)/NADPH redox couple is essential for cellular metabolism and redox homeostasis. However, how the homeostasis of these two dinucleotides is integrated into chloroplast biogenesis remains largely unknown. Here, we demonstrate the important role of NADP(+) supply for the biogenesis of PSI by examining the nad kinase 2 (nadk2) mutant in Arabidopsis (Arabidopsis thaliana), which demonstrates disrupted synthesis of NADP(+) from NAD(+) in chloroplasts. Although the nadk2 mutant is highly sensitive to light, the reaction center of photosystem II (PSII) is only mildly and likely only secondarily affected compared to the wild-type. Our studies revealed that the primary limitation of photosynthetic electron transport, even at low light intensities, occurs at PSI rather than at PSII in the nadk2 mutant. Remarkably, this primarily impairs the de novo synthesis of the two PSI core subunits PsaA and PsaB, leading to the deficiency of the PSI complex in the nadk2 mutant. This study reveals an unexpected molecular link between NADK activity and mRNA translation of psaA/B in chloroplasts that may mediate a feedback mechanism to adjust de novo biosynthesis of the PSI complex in response to a variable NADPH demand. This adjustment may be important to protect PSI from photoinhibition under conditions that favor acceptor side limitation. Mutation of NAD KINASE2 leads to NADP+ deficiency and impairs the synthesis of photosystem I in chloroplasts of Arabidopsis.
学科领域	Plant Sciences
DOI	10.1093/plphys/kiac161
收录类别	SCI
语种	英语
WOS关键词	THYLAKOID MEMBRANE ; CHLOROPHYLL-FLUORESCENCE ; NAD KINASE ; PYRIDINE-NUCLEOTIDES ; PROTEIN ; FERREDOXIN ; BIOGENESIS ; PLANTS ; DOMAIN ; IDENTIFICATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000786134700001
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-2548
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDA26030202] ; National Key Research and Development Program [2020YFA0907601] ; National Science Foundation of China [31000339] ; German Science Foundation (DFG) [SFB TRR175]
作者邮箱	jidaili@ibcas.ac.cn ; chiweimr@ibcas.ac.cn
作品OA属性	Bronze, Green Published
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28545
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ludwig Maximilians Univ Munchen, Fac Biol, Plant Mol Biol, D-82152 Munich, Germany 4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Ji, Daili,Li, Qiuxin,Guo, Yinjie,et al. NADP+ supply adjusts the synthesis of photosystem I in Arabidopsis chloroplasts[J]. PLANT PHYSIOLOGY,2022,189(4):2128-2143.
APA	Ji, Daili.,Li, Qiuxin.,Guo, Yinjie.,An, Wenjing.,Manavski, Nikolay.,...&Chi, Wei.(2022).NADP+ supply adjusts the synthesis of photosystem I in Arabidopsis chloroplasts.PLANT PHYSIOLOGY,189(4),2128-2143.
MLA	Ji, Daili,et al."NADP+ supply adjusts the synthesis of photosystem I in Arabidopsis chloroplasts".PLANT PHYSIOLOGY 189.4(2022):2128-2143.

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