Ectopic expression of Populus MYB10 promotes secondary cell wall thickening and inhibits anthocyanin accumulation

doi:10.1016/j.plaphy.2022.01.003

	Ectopic expression of Populus MYB10 promotes secondary cell wall thickening and inhibits anthocyanin accumulation
	Jiang, Peng-Fei ; Lin, Xiao-Yang ; Bian, Xiu-Yan ; Zeng, Qing-Yin ; Liu, Yan-Jing
	2022
发表期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
卷号	172 页码:24-32
摘要	Secondary cell wall (SCW) formation is regulated by a multilevel transcriptional regulatory network, in which MYB transcription factors (TFs) play key roles. In woody plants, hundreds of MYB TFs have been identified, most of which have unknown functions in wood SCW biosynthesis. Here, we characterized the function of a Populus MYB gene, PtoMYB10. PtoMYB10 was found to encode an R2R3-MYB TF and exhibit dominant expression in xylem tissues. PtoMYB10 was determined to be located in the nucleus with the ability to activate transcription. Overexpression of PtoMYB10 in Populus resulted in a drastic increase in SCW thickening in xylem fiber cells as well as ectopic deposition of lignin in cortex cells. The expression of genes associated with lignin biosynthesis was induced in PtoMYB10 overexpressing plants, whereas repressed gene expression was found with the anthocyanin biosynthesis pathway. Lignin and anthocyanin are both produced from metabolites of the phe-nylpropanoid pathway. Accordingly, the anthocyanin content of Populus overexpressing PtoMYB10 decreased by more than 68%. These results indicate that PtoMYB10 can positively regulate xylary fiber SCW thickening, accompanied by the reprogramming of phenylpropanoid metabolism, which redirects metabolic flux from anthocyanin biosynthesis to monolignol biosynthesis.
关键词	Populus MYB10 Secondary cell wall biosynthesis Anthocyanin biosynthesis
学科领域	Plant Sciences
DOI	10.1016/j.plaphy.2022.01.003
收录类别	SCI
语种	英语
WOS关键词	WOOD FORMATION ; TRANSCRIPTION FACTORS ; POPLAR ; FAMILY ; GENES ; BIOSYNTHESIS ; TRICHOCARPA ; GENOME
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000783431500003
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
文献子类	Article
出版地	ISSY-LES-MOULINEAUX
EISSN	1873-2690
资助机构	National Natural Science Foundation of China [31822011] ; Fundamental Research Funds of Chinese Academy of Forestry [CAFYBB2018ZX001, CAFYBB2019ZY002] ; State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry [TGB2019001] ; (Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing)
作者邮箱	yan.jing.liu@163.com
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28558
专题	系统与进化植物学国家重点实验室
作者单位	1.[Jiang, Peng-Fei 2.Bian, Xiu-Yan 3.Zeng, Qing-Yin 4.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Peng-Fei,Lin, Xiao-Yang,Bian, Xiu-Yan,et al. Ectopic expression of Populus MYB10 promotes secondary cell wall thickening and inhibits anthocyanin accumulation[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2022,172:24-32.
APA	Jiang, Peng-Fei,Lin, Xiao-Yang,Bian, Xiu-Yan,Zeng, Qing-Yin,&Liu, Yan-Jing.(2022).Ectopic expression of Populus MYB10 promotes secondary cell wall thickening and inhibits anthocyanin accumulation.PLANT PHYSIOLOGY AND BIOCHEMISTRY,172,24-32.
MLA	Jiang, Peng-Fei,et al."Ectopic expression of Populus MYB10 promotes secondary cell wall thickening and inhibits anthocyanin accumulation".PLANT PHYSIOLOGY AND BIOCHEMISTRY 172(2022):24-32.

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