Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata

doi:10.1111/nph.17962

	Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata
	Yan, Huili ; Xu, Wenxiu ; Zhang, Tian 1; Feng, Lu ; Liu, Ruoxi 1; Wang, Luyao 1; Wu, Lulu 1; Zhang, Han 1; Zhang, Xiaohan 2; Li, Ting 1; Peng, Zhimei 1; Jin, Chen 2; Yu, Yijun 3; Ping, Junai 4; Ma, Mi ; He, Zhenyan
	2022
发表期刊	NEW PHYTOLOGIST
ISSN	0028-646X
卷号	233 期号:6 页码:2488-2502
摘要	Pteris vittata is an arsenic (As) hyperaccumulator that can accumulate several thousand mg As kg(-1) DW in aboveground biomass. A key factor for its hyperaccumulation ability is its highly efficient As long-distance translocation system. However, the underlying molecular mechanisms remain unknown. We isolated PvAsE1 through the full-length cDNA over-expression library of P. vittata and characterized it through a yeast system, RNAi gametophytes and sporophytes, subcellular-location and in situ hybridization. Phylogenomic analysis was conducted to estimate the appearance time of PvAsE1. PvAsE1 was a plasma membrane-oriented arsenite (AsIII) effluxer. The silencing of PvAsE1 reduced AsIII long-distance translocation in P. vittata sporophytes. PvAsE1 was structurally similar to solute carrier (SLC)13 proteins. Its transcripts could be observed in parenchyma cells surrounding the xylem of roots. The appearance time was estimated at c. 52.7 Ma. PvAsE1 was a previously uncharacterized SLC13-like AsIII effluxer, which may contribute to AsIII long-distance translocation via xylem loading. PvAsE1 appeared late in fern evolution and might be an adaptive subject to the selection pressure at the Cretaceaou-Paleogene boundary. The identification of PvAsE1 provides clues for revealing the special As hyperaccumulation characteristics of P. vittata.
关键词	AsIII efflux long-distance translocation Pteris vittata PvAsE1 SLC13-like protein
学科领域	Plant Sciences
DOI	10.1111/nph.17962
收录类别	SCI
语种	英语
WOS关键词	CRETACEOUS-TERTIARY EXTINCTION ; DICARBOXYLATE CARRIER PROTEIN ; FUNCTIONAL-CHARACTERIZATION ; ACCUMULATION ; TOLERANCE ; EFFICIENT ; MECHANISMS ; SILICON ; RICE ; PHYTOREMEDIATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000748358900001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1469-8137
资助机构	National Natural Science Foundation of China [31770298, 41977131] ; National Key Research and Development Program of China [2017YFD0800900] ; Innovation Training Programs for Undergraduates, CAS [20214000859]
作者邮箱	hezhenyan@ibcas.ac.cn
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28560
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China 4.Zhejiang Stn Management Arable Land Qual & Fertil, Hangzhou 310020, Peoples R China 5.Shanxi Agr Univ, Sorghum Res Inst, Jinzhong 030600, Peoples R China
推荐引用方式 GB/T 7714	Yan, Huili,Xu, Wenxiu,Zhang, Tian,et al. Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata[J]. NEW PHYTOLOGIST,2022,233(6):2488-2502.
APA	Yan, Huili.,Xu, Wenxiu.,Zhang, Tian.,Feng, Lu.,Liu, Ruoxi.,...&He, Zhenyan.(2022).Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata.NEW PHYTOLOGIST,233(6),2488-2502.
MLA	Yan, Huili,et al."Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata".NEW PHYTOLOGIST 233.6(2022):2488-2502.

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