Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat

doi:10.1007/s00122-022-04184-y

	Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat
	Jiang, Hao ; Fang, Yu ; Yan, Dong ; Liu, Si-tong ; Wei, Jun ; Guo, Fei-long ; Wu, Xing-ting ; Cao, Hong ; Yin, Chang-bin ; Lu, Fei 3,4; Gao, Li-feng ; Liu, Yong-xiu
	2022
发表期刊	THEORETICAL AND APPLIED GENETICS
ISSN	0040-5752
卷号	135 期号:9 页码:3265-3276
摘要	Key message Twelve QTL associated with pre-harvest sprouting tolerance were identified using association analysis in wheat. Two markers were validated and a candidate gene TaNAC074 for Qgpf.cas-3B.2 was verified using Agrobacterium-mediated transformation. Pre-harvest sprouting (PHS) is a considerable global threat to wheat yield and quality. Due to this threat, breeders must identify quantitative trait loci (QTL) and genes conferring PHS-tolerance (PHST) to reduce the negative effects of PHS caused by low seed dormancy. In this study, we evaluated a panel of 302 diverse wheat genotypes for PHST in four environments and genotyped the panel with a high-density wheat 660 K SNP array. By using a genome-wide association study (GWAS), we identified 12 stable loci significantly associated with PHST (P < 0.0001), explaining 3.34 - 9.88% of the phenotypic variances. Seven of these loci co-located with QTL and genes reported previously. Five loci (Qgpf.cas-3B.2, Qgpf.cas-3B.3, Qgpf.cas-3B.4, Qgpf.cas-7B.2, and Qgpf.cas-7B.3), located in genomic regions with no known PHST QTL or genes, are likely to be new QTL conferring PHST. Additionally, two molecular markers were developed for Qgpf.cas-3A and Qgpf.cas-7B.3, and validated using a different set of 233 wheat accessions. Finally, the PHST-related function of candidate gene TaNAC074 for Qgpf.cas-3B.2 was confirmed by CAPS (cleaved amplified polymorphic sequences) marker association analysis in 233 wheat accessions and by expression and phenotypic analysis of transgenic wheat. Overexpression of TaNAC074 significantly reduced seed dormancy in wheat. This study contributes to broaden the genetic basis and molecular marker-assisted breeding of PHST.
学科领域	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
DOI	10.1007/s00122-022-04184-y
收录类别	SCI
语种	英语
WOS关键词	QUANTITATIVE TRAIT LOCI ; SEED-GERMINATION ; RESISTANCE ; DORMANCY ; GENES ; SELECTION ; CLONING ; FAMILY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000830275100001
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1432-2242
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010104] ; National Natural Science Foundation of China [32001431, U20A2033, 31870305]
作者邮箱	gaolifeng@caas.cn ; yongxiu@ibcas.ac.cn
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28591
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Gene Resources & Germplasm Enhanceme, Beijing 100081, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 5.Chinese Acad Sci, CAS JIC Ctr Excellence Plant & Microbial Sci CEPA, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Hao,Fang, Yu,Yan, Dong,et al. Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat[J]. THEORETICAL AND APPLIED GENETICS,2022,135(9):3265-3276.
APA	Jiang, Hao.,Fang, Yu.,Yan, Dong.,Liu, Si-tong.,Wei, Jun.,...&Liu, Yong-xiu.(2022).Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat.THEORETICAL AND APPLIED GENETICS,135(9),3265-3276.
MLA	Jiang, Hao,et al."Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat".THEORETICAL AND APPLIED GENETICS 135.9(2022):3265-3276.

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