The plastid-encoded protein Orf2971 is required for protein translocation and chloroplast quality control

doi:10.1093/plcell/koac180

IB-CAS > 中科院光生物学重点实验室

	The plastid-encoded protein Orf2971 is required for protein translocation and chloroplast quality control
	Xing, Jiale 1; Pan, Junting 1; Yi, Heng 1; Lv, Kang 1; Gan, Qiuliang 1; Wang, Meimei 1; Ge, Haitao 2; Huang, Xiahe 2; Huang, Fang; Wang, Yingchun 2; Rochaix, Jean-David ; Yang, Wenqiang1,5,6
	2022
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	34 期号:9 页码:3383-3399
摘要	Photosynthesis and the biosynthesis of many important metabolites occur in chloroplasts. In these semi-autonomous organelles, the chloroplast genome encodes approximately 100 proteins. The remaining chloroplast proteins, close to 3,000, are encoded by nuclear genes whose products are translated in the cytosol and imported into chloroplasts. However, there is still no consensus on the composition of the protein import machinery including its motor proteins and on how newly imported chloroplast proteins are refolded. In this study, we have examined the function of orf2971, the largest chloroplast gene of Chlamydomonas reinhardtii. The depletion of Orf2971 causes the accumulation of protein precursors, partial proteolysis and aggregation of proteins, increased expression of chaperones and proteases, and autophagy. Orf2971 interacts with the TIC (translocon at the inner chloroplast envelope) complex, catalyzes ATP (adenosine triphosphate) hydrolysis, and associates with chaperones and chaperonins. We propose that Orf2971 is intimately connected to the protein import machinery and plays an important role in chloroplast protein quality control. Repression of Orf2971 induces accumulation of chloroplast precursor proteins and impaired chloroplast quality indicating that Orf2971 is required for protein import and chloroplast quality control.
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1093/plcell/koac180
收录类别	SCI
语种	英语
WOS关键词	OPEN READING FRAMES ; CHLAMYDOMONAS-REINHARDTII ; CHAPERONIN 60 ; AUTOPHAGY ; EXPRESSION ; REVEALS ; IMPORT ; GENE ; TRANSFORMATION ; ORGANIZATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000817126000001
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-298X
资助机构	National Key R&D Program of China [2019YFA0906300] ; National Natural Science Foundation of China [31870217]
作者邮箱	wqyang@ibcas.ac.cn
作品OA属性	Green Published, hybrid
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/28595
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China 4.Univ Geneva, Dept Mol Biol, Geneva, Switzerland 5.Univ Geneva, Dept Plant Biol, Geneva, Switzerland 6.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China 7.Univ Chinese Acad Sci, Sino Danish Coll, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xing, Jiale,Pan, Junting,Yi, Heng,et al. The plastid-encoded protein Orf2971 is required for protein translocation and chloroplast quality control[J]. PLANT CELL,2022,34(9):3383-3399.
APA	Xing, Jiale.,Pan, Junting.,Yi, Heng.,Lv, Kang.,Gan, Qiuliang.,...&Yang, Wenqiang.(2022).The plastid-encoded protein Orf2971 is required for protein translocation and chloroplast quality control.PLANT CELL,34(9),3383-3399.
MLA	Xing, Jiale,et al."The plastid-encoded protein Orf2971 is required for protein translocation and chloroplast quality control".PLANT CELL 34.9(2022):3383-3399.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Xing-2022-The plasti（2282KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文